Human CD300F ELISA Kit PicoKine®

CMRF35-like molecule 1 is a protein that in humans is encoded by the CD300LF gene. It is mapped to 17q25.1. This gene encodes a member of the CD300 protein family. Members of this family are cell surface glycoproteins with a single IgV-like extracellular domain, and are involved in the regulation of immune response. The encoded protein is an inhibitory receptor. Alternative splicing results in multiple transcript variants.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 60 53 52 60 56 3.76 6.7% Sample 2 272 252 233 247 251 13.98 5.5% Sample 3 844 800 822 904 842 38.76 4.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 60 53 52 60 56 3.76 6.7% Sample 1 60 53 52 60 56 3.76 6.7% Sample 2 272 252 233 247 251 13.98 5.5% Sample 2 272 252 233 247 251 13.98 5.5% Sample 3 844 800 822 904 842 38.76 4.6% Sample 3 844 800 822 904 842 38.76 4.6%

CMRF35-like molecule 1; CLM-1; CD300 antigen-like family member F; Immune receptor expressed on myeloid cells 1; IREM-1; Immunoglobulin superfamily member 13; IgSF13; NK inhibitory receptor; CD300f; CD300LF; CD300F; CLM1; IGSF13; IREM1; NKIR; UNQ3105/PRO1

Highly expressed in spleen, peripheral blood leukocyte and monocyte, and lung. Weakly expressed in thymus, heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, testis, ovary, small intestine or colon. Expressed selectively in monocy

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 60 272 844 67 385 987 Standard deviation 3.72 14.42 39.67 4.09 28.49 50.34 CV (%) 6.2% 5.3% 4.7% 6.1% 7.4% 5.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 60 272 844 67 385 987 Standard deviation 3.72 14.42 39.67 4.09 28.49 50.34 CV (%) 6.2% 5.3% 4.7% 6.1% 7.4% 5.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 60 272 844 67 385 987 Mean (pg/ml) 60 272 844 67 385 987 Standard deviation 3.72 14.42 39.67 4.09 28.49 50.34 Standard deviation 3.72 14.42 39.67 4.09 28.49 50.34 CV (%) 6.2% 5.3% 4.7% 6.1% 7.4% 5.1% CV (%) 6.2% 5.3% 4.7% 6.1% 7.4% 5.1%

Acts as an inhibitory receptor for myeloid cells and mast cells. Positively regulates the phagocytosis of apoptotic cells (efferocytosis) via phosphatidylserine (PS) recognition; recognizes and binds PS as a ligand which is expressed on the surface of apo

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.059 0.101 0.144 0.214 0.394 0.704 1.288 2.302 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.059 0.101 0.144 0.214 0.394 0.704 1.288 2.302 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.059 0.101 0.144 0.214 0.394 0.704 1.288 2.302 O.D. 0.059 0.101 0.144 0.214 0.394 0.704 1.288 2.302

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.