Mouse FCRL1 ELISA Kit PicoKine®

Mouse FCRL1 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Fcrla in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Fc receptor-like protein 1 is a protein that in humans is encoded by the FCRL1 gene. It is mapped to 3; 3 F1.This gene encodes a member of the immunoglobulin receptor superfamily and is one of several Fc receptor-like glycoproteins clustered on the long arm of chromosome 1. The encoded protein contains three extracellular C2-like immunoglobulin domains, a transmembrane domain and a cytoplasmic domain with two immunoreceptor-tyrosine activation motifs. This protein may play a role in the regulation of cancer cell growth. Alternative splicing results in multiple transcript variants.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 90 96 85 88 89 4.02 4.5% Sample 2 464 409 476 420 442 28.34 6.4% Sample 3 1704 1617 1595 1596 1628 44.74 2.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 90 96 85 88 89 4.02 4.5% Sample 1 90 96 85 88 89 4.02 4.5% Sample 2 464 409 476 420 442 28.34 6.4% Sample 2 464 409 476 420 442 28.34 6.4% Sample 3 1704 1617 1595 1596 1628 44.74 2.7% Sample 3 1704 1617 1595 1596 1628 44.74 2.7%

Synonyms

Fc receptor-like A; Fc receptor homolog expressed in B-cells; Fc receptor-like and mucin-like protein 1; Fc receptor-like protein; Fc receptor-related protein X; FcRX; Fcrla; Fcrl; Fcrl1; Fcrlm1; Fcrx; Freb

Gene Name

Fcrla

Gene ID

229499

UniProt

Q920A9

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A17-S219

Capture Antibody

A monoclonal antibody from rat

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Tissue Specificity

Highly expressed in spleen. Expressed in immature B-cell and B-cell lines.

Applications

ELISA

Field of Research

Adaptive Immunity, B Cells, Immunoglobulins, Immunology, Non-CD, Receptors

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 90 464 1704 96 476 1814 Standard deviation 4.41 21.81 69.86 5.38 24.28 83.44 CV (%) 4.9% 4.7% 4.1% 5.6% 5.1% 4.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 90 464 1704 96 476 1814 Standard deviation 4.41 21.81 69.86 5.38 24.28 83.44 CV (%) 4.9% 4.7% 4.1% 5.6% 5.1% 4.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 90 464 1704 96 476 1814 Mean (pg/ml) 90 464 1704 96 476 1814 Standard deviation 4.41 21.81 69.86 5.38 24.28 83.44 Standard deviation 4.41 21.81 69.86 5.38 24.28 83.44 CV (%) 4.9% 4.7% 4.1% 5.6% 5.1% 4.6% CV (%) 4.9% 4.7% 4.1% 5.6% 5.1% 4.6%

Sensitivity

<50 pg/mL

Function

May be implicated in B-cell differentiation and lymphomagenesis.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse FCRL1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Fc receptor-like 1

Subcellular Location

Cytoplasm.

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.067 0.111 0.147 0.231 0.391 0.7 1.345 2.226 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.067 0.111 0.147 0.231 0.391 0.7 1.345 2.226 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.067 0.111 0.147 0.231 0.391 0.7 1.345 2.226 O.D. 0.067 0.111 0.147 0.231 0.391 0.7 1.345 2.226

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.