Mouse FCRL5 ELISA Kit PicoKine®

Mouse FCRL5 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse FCRL5 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Fc receptor-like protein 5 is a protein that in humans is encoded by the FCRL5 gene. It is mapped to 3; 3 F1. FCRL5 has also been designated as CD307 (cluster of differentiation 307) .

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 18 22 28 29 24 1.62 6.7% Sample 2 72 78 85 92 82 4.33 5.3% Sample 3 133 145 1.57 144 145 7.09 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 18 22 28 29 24 1.62 6.7% Sample 1 18 22 28 29 24 1.62 6.7% Sample 2 72 78 85 92 82 4.33 5.3% Sample 2 72 78 85 92 82 4.33 5.3% Sample 3 133 145 1.57 144 145 7.09 4.9% Sample 3 133 145 1.57 144 145 7.09 4.9%

Synonyms

Fc receptor-like protein 5; FcR-like protein 5; FcRL5; BXMAS1-like protein 2; Mbxmh2; Fc receptor homolog 3; FcRH3; moFcRH3; CD307e; CD307e; Fcrl5; Fcrh3

Gene Name

FCRL5

Gene ID

329693

UniProt

Q68SN8

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0, Immunogen sequence: Q27-A496

Capture Antibody

A monoclonal antibody from rat

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Tissue Specificity

Preferentially expressed in marginal zone B cells.

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 18 72 133 22 85 154 Standard deviation 1.06 3.17 8.38 1.01 4.93 11.55 CV (%) 5.9% 4.4% 6.3% 4.6% 5.8% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 18 72 133 22 85 154 Standard deviation 1.06 3.17 8.38 1.01 4.93 11.55 CV (%) 5.9% 4.4% 6.3% 4.6% 5.8% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 18 72 133 22 85 154 Mean (pg/ml) 18 72 133 22 85 154 Standard deviation 1.06 3.17 8.38 1.01 4.93 11.55 Standard deviation 1.06 3.17 8.38 1.01 4.93 11.55 CV (%) 5.9% 4.4% 6.3% 4.6% 5.8% 7.5% CV (%) 5.9% 4.4% 6.3% 4.6% 5.8% 7.5%

Sensitivity

<10 pg/mL

Function

It is uncertain whether Met-1 or Met-14 is the initiator.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse FCRL5

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Fc receptor-like 5

Subcellular Location

Cell membrane. Single-pass type I membrane protein.

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.042 0.1 0.155 0.274 0.472 0.874 1.543 2.576 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.042 0.1 0.155 0.274 0.472 0.874 1.543 2.576 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.042 0.1 0.155 0.274 0.472 0.874 1.543 2.576 O.D. 0.042 0.1 0.155 0.274 0.472 0.874 1.543 2.576

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.