Human FCRL2 ELISA Kit PicoKine®

Fc receptor-like protein 2 is a protein that in humans is encoded by the FCRL2 gene. It is mapped to 1q23.1. This gene encodes a member of the immunoglobulin receptor superfamily and is one of several Fc receptor-like glycoproteins clustered on the long arm of chromosome 1. The encoded protein has four extracellular C2-type immunoglobulin domains, a transmembrane domain and a cytoplasmic domain that contains one immunoreceptor-tyrosine activation motif and two immunoreceptor-tyrosine inhibitory motifs. This protein may be a prognostic marker for chronic lymphocytic leukemia. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 44 50 48 59 50 2.56 5.1% Sample 2 136 152 144 146 145 4.77 3.3% Sample 3 578 602 594 585 590 45.59 7.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 44 50 48 59 50 2.56 5.1% Sample 1 44 50 48 59 50 2.56 5.1% Sample 2 136 152 144 146 145 4.77 3.3% Sample 2 136 152 144 146 145 4.77 3.3% Sample 3 578 602 594 585 590 45.59 7.9% Sample 3 578 602 594 585 590 45.59 7.9%

Fc receptor-like protein 2; FcR-like protein 2; FcRL2; Fc receptor homolog 2; FcRH2; IFGP family protein 4; Immunoglobulin receptor translocation-associated protein 4; SH2 domain-containing phosphatase anchor protein 1; CD307b; FCRL2; FCRH2; IFGP4; IRTA4

Expressed in the secondary lymphoid organs, spleen and lymph node. Expression is limited to the mature B-cell lines. Highly expressed in CD19 and within the mantle zones of the tonsil tissue. Isoform 2 is expressed in the spleen, peripheral blood and bone

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 44 136 578 53 168 605 Standard deviation 3.17 8.57 37.17 2.39 7.06 32.07 CV (%) 7.2% 6.3% 6.4% 4.5% 4.2% 5.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 44 136 578 53 168 605 Standard deviation 3.17 8.57 37.17 2.39 7.06 32.07 CV (%) 7.2% 6.3% 6.4% 4.5% 4.2% 5.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 44 136 578 53 168 605 Mean (pg/ml) 44 136 578 53 168 605 Standard deviation 3.17 8.57 37.17 2.39 7.06 32.07 Standard deviation 3.17 8.57 37.17 2.39 7.06 32.07 CV (%) 7.2% 6.3% 6.4% 4.5% 4.2% 5.3% CV (%) 7.2% 6.3% 6.4% 4.5% 4.2% 5.3%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.016 0.05 0.088 0.161 0.337 0.734 1.568 2.844 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.016 0.05 0.088 0.161 0.337 0.734 1.568 2.844 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.016 0.05 0.088 0.161 0.337 0.734 1.568 2.844 O.D. 0.016 0.05 0.088 0.161 0.337 0.734 1.568 2.844

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.