Human FLRT3 ELISA Kit PicoKine®

Leucine-rich repeat transmembrane protein FLRT3 is a protein that in humans is encoded by the FLRT3 gene. It is mapped to 20p12.1. This gene encodes a member of the fibronectin leucine rich transmembrane protein (FLRT) family. FLRTs may function in cell adhesion and/or receptor signalling. Their protein structures resemble small leucine-rich proteoglycans found in the extracellular matrix. This gene is expressed in many tissues. Two alternatively spliced transcript variants encoding the same protein have been described for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 206 228 212 203 212 9.65 4.5% Sample 2 1152 1104 1147 1147 1137 19.44 1.7% Sample 3 4318 3995 4413 3635 4090 305.10 7.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 206 228 212 203 212 9.65 4.5% Sample 1 206 228 212 203 212 9.65 4.5% Sample 2 1152 1104 1147 1147 1137 19.44 1.7% Sample 2 1152 1104 1147 1147 1137 19.44 1.7% Sample 3 4318 3995 4413 3635 4090 305.10 7.4% Sample 3 4318 3995 4413 3635 4090 305.10 7.4%

Leucine-rich repeat transmembrane protein FLRT3; Fibronectin-like domain-containing leucine-rich transmembrane protein 3; FLRT3; KIAA1469; UNQ856/PRO1865

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 206 1152 4318 228 1236 4423 Standard deviation 12.15 49.54 220.22 15.05 71.69 203.46 CV (%) 5.9% 4.3% 5.1% 6.6% 5.8% 4.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 206 1152 4318 228 1236 4423 Standard deviation 12.15 49.54 220.22 15.05 71.69 203.46 CV (%) 5.9% 4.3% 5.1% 6.6% 5.8% 4.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 206 1152 4318 228 1236 4423 Mean (pg/ml) 206 1152 4318 228 1236 4423 Standard deviation 12.15 49.54 220.22 15.05 71.69 203.46 Standard deviation 12.15 49.54 220.22 15.05 71.69 203.46 CV (%) 5.9% 4.3% 5.1% 6.6% 5.8% 4.6% CV (%) 5.9% 4.3% 5.1% 6.6% 5.8% 4.6%

Functions in cell-cell adhesion, cell migration and axon guidance, exerting an attractive or repulsive role depending on its interaction partners. Plays a role in the spatial organization of brain neurons. Plays a role in vascular development in the retin

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.037 0.103 0.173 0.29 0.543 0.989 1.578 2.092 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.037 0.103 0.173 0.29 0.543 0.989 1.578 2.092 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.037 0.103 0.173 0.29 0.543 0.989 1.578 2.092 O.D. 0.037 0.103 0.173 0.29 0.543 0.989 1.578 2.092

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.