Rat FLRT2 ELISA Kit PicoKine®

Fibronectin leucine-rich repeat transmembrane protein FLRT2 is a protein that in humans is encoded by the FLRT2 gene. It is mapped to 6q31. This gene encodes a member of the fibronectin leucine rich transmembrane (FLRT) family of cell adhesion molecules, which regulate early embryonic vascular and neural development. The encoded type I transmembrane protein has an extracellular region consisting of an N-terminal leucine-rich repeat domain and a type 3 fibronectin domain, followed by a transmembrane domain and a short C-terminal cytoplasmic tail domain. It functions as both a homophilic cell adhesion molecule and a heterophilic chemorepellent through its interaction with members of the uncoordinated-5 receptor family. Proteolytic removal of the extracellular region controls the migration of neurons in the developing cortex. Alternative splicing results in multiple transcript variants.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 53 50 55 51 52 1.92 3.6% Sample 2 293 280 261 256 272 14.84 5.4% Sample 3 951 833 887 863 883 43.41 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 53 50 55 51 52 1.92 3.6% Sample 1 53 50 55 51 52 1.92 3.6% Sample 2 293 280 261 256 272 14.84 5.4% Sample 2 293 280 261 256 272 14.84 5.4% Sample 3 951 833 887 863 883 43.41 4.9% Sample 3 951 833 887 863 883 43.41 4.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 53 293 951 55 295 959 Standard deviation 2.44 14.36 53.26 3.74 15.34 56.58 CV (%) 4.6% 4.9% 5.6% 6.8% 5.2% 5.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 53 293 951 55 295 959 Standard deviation 2.44 14.36 53.26 3.74 15.34 56.58 CV (%) 4.6% 4.9% 5.6% 6.8% 5.2% 5.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 53 293 951 55 295 959 Mean (pg/ml) 53 293 951 55 295 959 Standard deviation 2.44 14.36 53.26 3.74 15.34 56.58 Standard deviation 2.44 14.36 53.26 3.74 15.34 56.58 CV (%) 4.6% 4.9% 5.6% 6.8% 5.2% 5.9% CV (%) 4.6% 4.9% 5.6% 6.8% 5.2% 5.9%

Functions in cell-cell adhesion, cell migration and axon guidance. Mediates cell-cell adhesion via its interactions with ADGRL3 and probably also other latrophilins that are expressed at the surface of adjacent cells. May play a role in the migration of c

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Extracellular matrix. Secreted. Endoplasmic reticulum membrane. Microsome membrane. Cell membrane. Single-pass membrane protein. Focal adhesion. Synaptosome.

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.037 0.098 0.143 0.265 0.473 0.887 1.678 3.012 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.037 0.098 0.143 0.265 0.473 0.887 1.678 3.012 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.037 0.098 0.143 0.265 0.473 0.887 1.678 3.012 O.D. 0.037 0.098 0.143 0.265 0.473 0.887 1.678 3.012

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.