Human PROC/Protein C ELISA Kit PicoKine®

Human PROC/Protein C ELISA Kit PicoKine® (96 Tests) . Quantitate Human Proc in cell culture supernatants, serum and plasma (heparin, EDTA) and urine. Sensitivity: 0.1ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Protein C (PROC), also called PC, is a zymogenic (inactive) protein, the activated form of which plays an important role in regulating blood clotting, inflammation, cell death and maintaining the permeability of blood vessel walls in humans and other animals. The PROC gene is mapped to 2q14.3. This gene encodes a vitamin K-dependent plasma glycoprotein. The encoded protein is cleaved to its activated form by the thrombinthrombomodulin complex. This activated form contains a serine protease domain and functions in degradation of the activated forms of coagulation factors V and VIII. Mutations in this gene have been associated with thrombophilia due to protein C deficiency, neonatal purpura fulminans, and recurrent venous thrombosis.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1223 1177 1347 1353 1275 76.77 6.0% Sample 2 5529 5748 6539 6544 6090 458.09 7.5% Sample 3 25231 23258 22794 25529 24203 1193.03 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1223 1177 1347 1353 1275 76.77 6.0% Sample 1 1223 1177 1347 1353 1275 76.77 6.0% Sample 2 5529 5748 6539 6544 6090 458.09 7.5% Sample 2 5529 5748 6539 6544 6090 458.09 7.5% Sample 3 25231 23258 22794 25529 24203 1193.03 4.9% Sample 3 25231 23258 22794 25529 24203 1193.03 4.9%

Synonyms

Vitamin K-dependent protein C; Anticoagulant protein C; Autoprothrombin IIA; Blood coagulation factor XIV; Proc

Gene Name

Proc

Gene ID

5624

UniProt

P33587

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A43-P461

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Tissue Specificity

Plasma; synthesized in the liver.

Applications

ELISA

Field of Research

Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Signal Transduction

Standard

50 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) and urine.

Detection Range

0.78 ng/mL - 50 ng/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1223 5529 25231 1347 6539 25258 Standard deviation 52.59 359.39 1185.86 83.51 320.41 1793.32 CV (%) 4.3% 6.5% 4.7% 6.2% 4.9% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1223 5529 25231 1347 6539 25258 Standard deviation 52.59 359.39 1185.86 83.51 320.41 1793.32 CV (%) 4.3% 6.5% 4.7% 6.2% 4.9% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1223 5529 25231 1347 6539 25258 Mean (pg/ml) 1223 5529 25231 1347 6539 25258 Standard deviation 52.59 359.39 1185.86 83.51 320.41 1793.32 Standard deviation 52.59 359.39 1185.86 83.51 320.41 1793.32 CV (%) 4.3% 6.5% 4.7% 6.2% 4.9% 7.1% CV (%) 4.3% 6.5% 4.7% 6.2% 4.9% 7.1%

Sensitivity

<0.1 ng/mL

Function

Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids. Exerts a protective effect on the endothelial cell barrier function.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human PROC/Protein C

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Protein C, inactivator of coagulation factors Va and VIIIa

Subcellular Location

Secreted. Endoplasmic reticulum. Golgi apparatus.

Sample Data

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.033 0.081 0.13 0.22 0.408 0.793 1.392 2.219 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.033 0.081 0.13 0.22 0.408 0.793 1.392 2.219 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.033 0.081 0.13 0.22 0.408 0.793 1.392 2.219 O.D. 0.033 0.081 0.13 0.22 0.408 0.793 1.392 2.219

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.