Rat Serpin D1 ELISA Kit PicoKine®

Heparin cofactor II (HCII), a protein encoded by the SERPIND1 gene, is a coagulation factor that inhibits IIa, and is a cofactor for heparin and dermatan sulfate. It is mapped to 11q23. This gene belongs to the serpin gene superfamily. Serpins play roles in many processes including inflammation, blood clotting, and cancer metastasis. Members of this family have highly conserved secondary structures with a reactive center loop that interacts with the protease active site to inhibit protease activity. This gene encodes a plasma serine protease that functions as a thrombin and chymotrypsin inhibitor. The protein is activated by heparin, dermatan sulfate, and glycosaminoglycans. Allelic variations in this gene are associated with heparin cofactor II deficiency.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1439 1508 1569 1379 1473 71.46 4.8% Sample 2 5322 4966 4804 4757 4962 221.70 4.4% Sample 3 21230 23126 20846 19667 21217 1243.44 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1439 1508 1569 1379 1473 71.46 4.8% Sample 1 1439 1508 1569 1379 1473 71.46 4.8% Sample 2 5322 4966 4804 4757 4962 221.70 4.4% Sample 2 5322 4966 4804 4757 4962 221.70 4.4% Sample 3 21230 23126 20846 19667 21217 1243.44 5.8% Sample 3 21230 23126 20846 19667 21217 1243.44 5.8%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1439 5322 21230 1508 5457 23126 Standard deviation 83.46 340.61 1379.95 72.38 398.36 1086.92 CV (%) 5.8% 6.4% 6.5% 4.8% 7.3% 4.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1439 5322 21230 1508 5457 23126 Standard deviation 83.46 340.61 1379.95 72.38 398.36 1086.92 CV (%) 5.8% 6.4% 6.5% 4.8% 7.3% 4.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1439 5322 21230 1508 5457 23126 Mean (pg/ml) 1439 5322 21230 1508 5457 23126 Standard deviation 83.46 340.61 1379.95 72.38 398.36 1086.92 Standard deviation 83.46 340.61 1379.95 72.38 398.36 1086.92 CV (%) 5.8% 6.4% 6.5% 4.8% 7.3% 4.7% CV (%) 5.8% 6.4% 6.5% 4.8% 7.3% 4.7%

Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT) .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.013 0.092 0.18 0.34 0.642 1.107 1.836 2.559 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.013 0.092 0.18 0.34 0.642 1.107 1.836 2.559 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.013 0.092 0.18 0.34 0.642 1.107 1.836 2.559 O.D. 0.013 0.092 0.18 0.34 0.642 1.107 1.836 2.559

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.