Human Serpin D1 ELISA Kit PicoKine®

Heparin cofactor II (HCII), a protein encoded by the SERPIND1 gene, is a coagulation factor that inhibits IIa, and is a cofactor for heparin and dermatan sulfate. It is mapped to 22q11.21. This gene belongs to the serpin gene superfamily. Serpins play roles in many processes including inflammation, blood clotting, and cancer metastasis. Members of this family have highly conserved secondary structures with a reactive center loop that interacts with the protease active site to inhibit protease activity. This gene encodes a plasma serine protease that functions as a thrombin and chymotrypsin inhibitor. The protein is activated by heparin, dermatan sulfate, and glycosaminoglycans. Allelic variations in this gene are associated with heparin cofactor II deficiency.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 976 1160 971 1107 1053 82.18 7.8% Sample 2 6436 5740 5874 6193 6060 272.05 4.4% Sample 3 22337 22710 20531 21109 21671 885.79 4.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 976 1160 971 1107 1053 82.18 7.8% Sample 1 976 1160 971 1107 1053 82.18 7.8% Sample 2 6436 5740 5874 6193 6060 272.05 4.4% Sample 2 6436 5740 5874 6193 6060 272.05 4.4% Sample 3 22337 22710 20531 21109 21671 885.79 4.0% Sample 3 22337 22710 20531 21109 21671 885.79 4.0%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 976 6436 22337 1236 8549 22654 Standard deviation 539.04 302.49 1183.86 70.45 538.59 1449.86 CV (%) 4.0% 4.7% 5.3% 5.7% 6.3% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 976 6436 22337 1236 8549 22654 Standard deviation 539.04 302.49 1183.86 70.45 538.59 1449.86 CV (%) 4.0% 4.7% 5.3% 5.7% 6.3% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 976 6436 22337 1236 8549 22654 Mean (pg/ml) 976 6436 22337 1236 8549 22654 Standard deviation 539.04 302.49 1183.86 70.45 538.59 1449.86 Standard deviation 539.04 302.49 1183.86 70.45 538.59 1449.86 CV (%) 4.0% 4.7% 5.3% 5.7% 6.3% 6.4% CV (%) 4.0% 4.7% 5.3% 5.7% 6.3% 6.4%

Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT-III) . Also inhibits chymotrypsin, but in a glycosaminogly

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.036 0.089 0.122 0.214 0.404 0.712 1.255 1.968 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.036 0.089 0.122 0.214 0.404 0.712 1.255 1.968 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.036 0.089 0.122 0.214 0.404 0.712 1.255 1.968 O.D. 0.036 0.089 0.122 0.214 0.404 0.712 1.255 1.968

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.