Human Cystatin M/Cystatin E/CST6 ELISA Kit PicoKine®

Cystatin-M is a protein that in humans is encoded by the CST6 gene. It is mapped to 11q13.1. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. This gene encodes a cystatin from the type 2 family, which is down-regulated in metastatic breast tumor cells as compared to primary tumor cells. Loss of expression is likely associated with the progression of a primary tumor to a metastatic phenotype.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 79 79 75 76 77 1.78 2.3% Sample 2 382 396 405 372 388 12.67 3.2% Sample 3 1415 1318 1387 1330 1362 39.97 2.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 79 79 75 76 77 1.78 2.3% Sample 1 79 79 75 76 77 1.78 2.3% Sample 2 382 396 405 372 388 12.67 3.2% Sample 2 382 396 405 372 388 12.67 3.2% Sample 3 1415 1318 1387 1330 1362 39.97 2.9% Sample 3 1415 1318 1387 1330 1362 39.97 2.9%

Restricted to the stratum granulosum of normal skin, the stratum granulosum/spinosum of psoriatic skin, and the secretory coils of eccrine sweat glands. Low expression levels are found in the nasal cavity.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 79 382 1415 83 409 1442 Standard deviation 4.90 17.19 80.66 4.07 23.72 90.85 CV (%) 6.2% 4.5% 5.7% 4.9% 5.8% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 79 382 1415 83 409 1442 Standard deviation 4.90 17.19 80.66 4.07 23.72 90.85 CV (%) 6.2% 4.5% 5.7% 4.9% 5.8% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 79 382 1415 83 409 1442 Mean (pg/ml) 79 382 1415 83 409 1442 Standard deviation 4.90 17.19 80.66 4.07 23.72 90.85 Standard deviation 4.90 17.19 80.66 4.07 23.72 90.85 CV (%) 6.2% 4.5% 5.7% 4.9% 5.8% 6.3% CV (%) 6.2% 4.5% 5.7% 4.9% 5.8% 6.3%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.015 0.129 0.223 0.423 0.689 1.097 1.676 2.111 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.015 0.129 0.223 0.423 0.689 1.097 1.676 2.111 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.015 0.129 0.223 0.423 0.689 1.097 1.676 2.111 O.D. 0.015 0.129 0.223 0.423 0.689 1.097 1.676 2.111

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.