Human Cystatin D/CST5 ELISA Kit PicoKine®

Cystatin-D is a protein that in humans is encoded by the CST5 gene. It is mapped to 20p11.21. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a protein found in saliva and tears. The encoded protein may play a protective role against proteinases present in the oral cavity.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 30 31 27 27 28 1.78 6.3% Sample 2 215 218 202 231 216 10.30 4.7% Sample 3 456 461 435 518 467 30.74 6.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 30 31 27 27 28 1.78 6.3% Sample 1 30 31 27 27 28 1.78 6.3% Sample 2 215 218 202 231 216 10.30 4.7% Sample 2 215 218 202 231 216 10.30 4.7% Sample 3 456 461 435 518 467 30.74 6.5% Sample 3 456 461 435 518 467 30.74 6.5%

Expressed in submandibular and sublingual saliva but not in parotid saliva. Expressed in parotid gland but not in seminal vesicle, prostate, epididymis, testis, ovary, placenta, thyroid, gastric corpus, small intestine, liver, or gall bladder tissue.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 30 215 456 31 287 532 Standard deviation 1.26 11.40 25.99 1.92 16.65 33.52 CV (%) 4.2% 5.3% 5.7% 6.2% 5.8% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 30 215 456 31 287 532 Standard deviation 1.26 11.40 25.99 1.92 16.65 33.52 CV (%) 4.2% 5.3% 5.7% 6.2% 5.8% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 30 215 456 31 287 532 Mean (pg/ml) 30 215 456 31 287 532 Standard deviation 1.26 11.40 25.99 1.92 16.65 33.52 Standard deviation 1.26 11.40 25.99 1.92 16.65 33.52 CV (%) 4.2% 5.3% 5.7% 6.2% 5.8% 6.3% CV (%) 4.2% 5.3% 5.7% 6.2% 5.8% 6.3%

Cysteine proteinase inhibitor that possibly plays a protective role against proteinases present in the oral cavity. The order of preference for inhibition is cathepsin S > cathepsin H > cathepsin L > cathepsin B.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.044 0.109 0.162 0.268 0.48 0.828 1.459 2.048 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.044 0.109 0.162 0.268 0.48 0.828 1.459 2.048 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.044 0.109 0.162 0.268 0.48 0.828 1.459 2.048 O.D. 0.044 0.109 0.162 0.268 0.48 0.828 1.459 2.048

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.