Human Serpin A6 ELISA Kit PicoKine®

Human Serpin A6 ELISA Kit PicoKine® (96 Tests) . Quantitate Human SERPINA6 in cell culture supernatants, serum and plasma (heparin, EDTA) and urine. Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Corticosteroid-binding globulin (CBG), also known as Serpin A6 is a protein that in humans is encoded by the SERPINA6 gene. It is mapped to 14q32.13. This gene encodes an alpha-globulin protein with corticosteroid-binding properties. This is the major transport protein for glucorticoids and progestins in the blood of most vertebrates. The gene localizes to a chromosomal region containing several closely related serine protease inhibitors which may have evolved by duplication events.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 2728 3062 2675 2954 2854 159.04 5.5% Sample 2 12106 13177 12457 12922 12665 413.50 3.2% Sample 3 43028 43427 45382 41055 43223 1536.32 3.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 2728 3062 2675 2954 2854 159.04 5.5% Sample 1 2728 3062 2675 2954 2854 159.04 5.5% Sample 2 12106 13177 12457 12922 12665 413.50 3.2% Sample 2 12106 13177 12457 12922 12665 413.50 3.2% Sample 3 43028 43427 45382 41055 43223 1536.32 3.5% Sample 3 43028 43427 45382 41055 43223 1536.32 3.5%

Synonyms

Corticosteroid-binding globulin; CBG; Serpin A6; Transcortin; SERPINA6; CBG

Gene Name

SERPINA6

Gene ID

866

UniProt

P08185

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: M23-V405

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Tissue Specificity

Plasma; synthesized in liver. Has also been identified in a number of glycocorticoid responsive cells.

Applications

ELISA

Field of Research

Cancer, Lipid Metabolism, Metabolism, Signal Transduction

Standard

100 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) and urine.

Detection Range

1.56 ng/mL - 100 ng/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2728 12106 43028 2986 12333 47148 Standard deviation 141.86 544.77 2452.60 206.03 715.31 2970.32 CV (%) 5.2% 4.5% 5.7% 6.9% 5.8% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2728 12106 43028 2986 12333 47148 Standard deviation 141.86 544.77 2452.60 206.03 715.31 2970.32 CV (%) 5.2% 4.5% 5.7% 6.9% 5.8% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 2728 12106 43028 2986 12333 47148 Mean (pg/ml) 2728 12106 43028 2986 12333 47148 Standard deviation 141.86 544.77 2452.60 206.03 715.31 2970.32 Standard deviation 141.86 544.77 2452.60 206.03 715.31 2970.32 CV (%) 5.2% 4.5% 5.7% 6.9% 5.8% 6.3% CV (%) 5.2% 4.5% 5.7% 6.9% 5.8% 6.3%

Sensitivity

<50 pg/mL

Function

Major transport protein for glucocorticoids and progestins in the blood of almost all vertebrate species.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human Serpin A6

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Serpin family A member 6

Subcellular Location

Secreted.

Sample Data

Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.02 0.089 0.151 0.251 0.489 0.862 1.653 2.48 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.02 0.089 0.151 0.251 0.489 0.862 1.653 2.48 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.02 0.089 0.151 0.251 0.489 0.862 1.653 2.48 O.D. 0.02 0.089 0.151 0.251 0.489 0.862 1.653 2.48

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.