Human URB/CCDC80 ELISA Kit PicoKine®

Coiled-coil domain-containing protein 80 is a protein that in humans is encoded by the CCDC80 gene. It is mapped to 3q13.2. Bioinformatics analysis suggests the CCDC80 protein is a peroxiredoxin.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 79 76 86 72 78 5.11 6.5% Sample2 359 370 404 430 390 28.08 7.2% Sample3 1459 1416 1530 1436 1460 43.04 2.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 79 76 86 72 78 5.11 6.5% Sample 1 79 76 86 72 78 5.11 6.5% Sample2 359 370 404 430 390 28.08 7.2% Sample2 359 370 404 430 390 28.08 7.2% Sample3 1459 1416 1530 1436 1460 43.04 2.9% Sample3 1459 1416 1530 1436 1460 43.04 2.9%

Coiled-coil domain-containing protein 80; Down-regulated by oncogenes protein 1; Up-regulated in BRS-3 deficient mouse homolog; CCDC80; DRO1; URB; HBE245

Expressed in dermal papilla and dermal fibroblasts (at protein level) . Expressed in heart, thymus, placenta, pancreas, colon, epithelium, spleen and osteoblasts.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 79 359 1459 86 438 1497 Standard deviation 3.32 24.05 80.25 5.85 25.84 91.32 CV (%) 4.2% 6.7% 5.5% 6.8% 5.9% 6.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 79 359 1459 86 438 1497 Standard deviation 3.32 24.05 80.25 5.85 25.84 91.32 CV (%) 4.2% 6.7% 5.5% 6.8% 5.9% 6.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 79 359 1459 86 438 1497 Mean (pg/ml) 79 359 1459 86 438 1497 Standard deviation 3.32 24.05 80.25 5.85 25.84 91.32 Standard deviation 3.32 24.05 80.25 5.85 25.84 91.32 CV (%) 4.2% 6.7% 5.5% 6.8% 5.9% 6.1% CV (%) 4.2% 6.7% 5.5% 6.8% 5.9% 6.1%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.021 0.086 0.145 0.276 0.535 1.014 1.701 2.472 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.021 0.086 0.145 0.276 0.535 1.014 1.701 2.472 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.021 0.086 0.145 0.276 0.535 1.014 1.701 2.472 O.D. 0.021 0.086 0.145 0.276 0.535 1.014 1.701 2.472

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.