Human Neudesin ELISA Kit PicoKine®

This gene encodes a neurotrophic factor that may play a role in neuron differentiation and development. A pseudogene of this gene is found on chromosome 12. Alternate splicing of this gene results in multiple transcript variants. It is mapped to 1q32.3.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 124 126 128 129 126 1.92 1.5% Sample 2 715 739 809 714 744 38.69 5.2% Sample 3 2078 2149 2030 2027 2071 49.37 2.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 124 126 128 129 126 1.92 1.5% Sample 1 124 126 128 129 126 1.92 1.5% Sample 2 715 739 809 714 744 38.69 5.2% Sample 2 715 739 809 714 744 38.69 5.2% Sample 3 2078 2149 2030 2027 2071 49.37 2.3% Sample 3 2078 2149 2030 2027 2071 49.37 2.3%

Neudesin; Cell immortalization-related protein 2; Neuron-derived neurotrophic factor; Protein GIG47; Secreted protein of unknown function; SPUF protein; NENF; CIR2; SPUF

Ubiquitously expressed with high expression in heart. Over-expressed in various tumors including carcinomas of the uterine cervix, lymphoma, colon, lung, skin and leukemia, as well as carcinoma of the breast.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 124 715 2078 159 739 2238 Standard deviation 7.69 32.18 118.45 7.79 42.86 140.99 CV (%) 6.2% 4.5% 5.7% 4.9% 5.8% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 124 715 2078 159 739 2238 Standard deviation 7.69 32.18 118.45 7.79 42.86 140.99 CV (%) 6.2% 4.5% 5.7% 4.9% 5.8% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 124 715 2078 159 739 2238 Mean (pg/ml) 124 715 2078 159 739 2238 Standard deviation 7.69 32.18 118.45 7.79 42.86 140.99 Standard deviation 7.69 32.18 118.45 7.79 42.86 140.99 CV (%) 6.2% 4.5% 5.7% 4.9% 5.8% 6.3% CV (%) 6.2% 4.5% 5.7% 4.9% 5.8% 6.3%

Acts as a neurotrophic factor in postnatal mature neurons enhancing neuronal survival. Promotes cell proliferation and neurogenesis in undifferentiated neural pro-genitor cells at the embryonic stage and inhibits differentiation of astrocyte. Its neurotrophic activity is exerted via MAPK1/ERK2, MAPK3/ERK1 and AKT1/AKT pathways. Neurotrophic activity is enhanced by binding to heme. Acts also as an anorexigenic neurotrophic factor that contributes to energy balance. Plays a role in the human tumorigenesis.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.066 0.107 0.159 0.255 0.449 0.795 1.426 2.116 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.066 0.107 0.159 0.255 0.449 0.795 1.426 2.116 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.066 0.107 0.159 0.255 0.449 0.795 1.426 2.116 O.D. 0.066 0.107 0.159 0.255 0.449 0.795 1.426 2.116

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.