Mouse CA4/Carbonic anhydrase 4 ELISA Kit PicoKine®

Carbonic anhydrase 4 is an enzyme that in humans is encoded by the CA4 gene. It is mapped to 11; 11 C. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IV is a glycosylphosphatidyl-inositol-anchored membrane isozyme expressed on the luminal surfaces of pulmonary (and certain other) capillaries and of proximal renal tubules. Its exact function is not known, however, it may have a role in inherited renal abnormalities of bicarbonate transport.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 135 147 139 153 143 6.98 4.8% Sample 2 612 690 628 619 637 30.97 4.8% Sample 3 2184 2286 2132 2361 2240 88.81 3.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 135 147 139 153 143 6.98 4.8% Sample 1 135 147 139 153 143 6.98 4.8% Sample 2 612 690 628 619 637 30.97 4.8% Sample 2 612 690 628 619 637 30.97 4.8% Sample 3 2184 2286 2132 2361 2240 88.81 3.9% Sample 3 2184 2286 2132 2361 2240 88.81 3.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 135 612 2184 140 618 2258 Standard deviation 7.02 38.56 104.83 6.86 45.73 119.67 CV (%) 5.2% 6.3% 4.8% 4.9% 7.4% 5.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 135 612 2184 140 618 2258 Standard deviation 7.02 38.56 104.83 6.86 45.73 119.67 CV (%) 5.2% 6.3% 4.8% 4.9% 7.4% 5.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 135 612 2184 140 618 2258 Mean (pg/ml) 135 612 2184 140 618 2258 Standard deviation 7.02 38.56 104.83 6.86 45.73 119.67 Standard deviation 7.02 38.56 104.83 6.86 45.73 119.67 CV (%) 5.2% 6.3% 4.8% 4.9% 7.4% 5.3% CV (%) 5.2% 6.3% 4.8% 4.9% 7.4% 5.3%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.002 0.114 0.205 0.357 0.631 1.056 1.661 2.095 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.002 0.114 0.205 0.357 0.631 1.056 1.661 2.095 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.002 0.114 0.205 0.357 0.631 1.056 1.661 2.095 O.D. 0.002 0.114 0.205 0.357 0.631 1.056 1.661 2.095

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.