Mouse CD200R1 ELISA Kit PicoKine®

Cell surface trasmembrane glycoprotein CD200 receptor 1 is a protein that in humans is encoded by the CD200R1 gene. It is mapped to 16; 16 B4. CD200R1 is expressed on the surface of myeloid cells and CD4+ T cells. It interacts with CD200 transmembrane glycoprotein that can be expressed on variety of cells including neurons, epithelial cells, endothelial cells, fibroblasts, and lymphoid cells CD200R1 activation regulates the expression of pro-inflammatory molecules such as tumor necrosis factor (TNF-alpha), interferons, and inducible nitric oxide synthase.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 128 115 126 129 124 5.59 4.5% Sample 2 681 595 579 675 632 45.89 7.2% Sample 3 2537 2458 2371 2185 2387 130.95 5.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 128 115 126 129 124 5.59 4.5% Sample 1 128 115 126 129 124 5.59 4.5% Sample 2 681 595 579 675 632 45.89 7.2% Sample 2 681 595 579 675 632 45.89 7.2% Sample 3 2537 2458 2371 2185 2387 130.95 5.4% Sample 3 2537 2458 2371 2185 2387 130.95 5.4%

Expressed in granulocytes, monocytes, most T-cells and a subset of NK, NKT and B-cells. Expressed in the spleen, lung, liver, testis, bone marrow, lymph nodes, spinal cord, kidney, uterus and small intestine. Expressed in mast and dendritic cells. Expressed in the lung of N. brasiliensis-infected mice.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 128 681 2537 139 708 3076 Standard deviation 5.76 44.27 169.98 9.45 41.77 187.64 CV (%) 4.5% 6.5% 6.7% 6.8% 5.9% 6.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 128 681 2537 139 708 3076 Standard deviation 5.76 44.27 169.98 9.45 41.77 187.64 CV (%) 4.5% 6.5% 6.7% 6.8% 5.9% 6.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 128 681 2537 139 708 3076 Mean (pg/ml) 128 681 2537 139 708 3076 Standard deviation 5.76 44.27 169.98 9.45 41.77 187.64 Standard deviation 5.76 44.27 169.98 9.45 41.77 187.64 CV (%) 4.5% 6.5% 6.7% 6.8% 5.9% 6.1% CV (%) 4.5% 6.5% 6.7% 6.8% 5.9% 6.1%

Inhibitory receptor for the CD200/OX2 cell surface glycoprotein. Limits inflammation by inhibiting the expression of proinflammatory molecules including TNF-alpha, interferons, and inducible nitric oxide synthase in response to selected stimuli.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.044 0.132 0.212 0.346 0.591 1.063 1.659 2.189 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.044 0.132 0.212 0.346 0.591 1.063 1.659 2.189 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.044 0.132 0.212 0.346 0.591 1.063 1.659 2.189 O.D. 0.044 0.132 0.212 0.346 0.591 1.063 1.659 2.189

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.