Human CA3 ELISA Kit PicoKine®

Human CA3 ELISA Kit PicoKine® (96 Tests) . Quantitate Human CA3 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) and urine. Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Carbonic anhydrase III (CA3) is an enzyme that in humans is encoded by the CA3 gene. CA3 is a member of a multigene family that encode carbonic anhydrase isozymes. It is mapped to 8q21.2. The gene spans 10.3 kb and contains seven exons and six introns. Using a cDNA clone of the CA3 gene in the study of human-rodent hybrids, the gene was mapped to chromosome 8 which carries a cluster of CA genes. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1301 1297 1136 1156 1222 76.83 6.2% Sample 2 6028 5779 6552 5475 5958 394.67 6.6% Sample 3 24574 24410 24517 21425 23731 1332.95 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1301 1297 1136 1156 1222 76.83 6.2% Sample 1 1301 1297 1136 1156 1222 76.83 6.2% Sample 2 6028 5779 6552 5475 5958 394.67 6.6% Sample 2 6028 5779 6552 5475 5958 394.67 6.6% Sample 3 24574 24410 24517 21425 23731 1332.95 5.6% Sample 3 24574 24410 24517 21425 23731 1332.95 5.6%

Synonyms

Carbonic anhydrase 3; Carbonate dehydratase III; Carbonic anhydrase III; CA-III; CA3

Gene Name

CA3

Gene ID

761

UniProt

P07451

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A2-K260

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Tissue Specificity

Muscle specific.

Applications

ELISA

Field of Research

Associated Proteins, Cardiovascular, Cell Biology, Cell Type Markers, Hypoxia

Standard

50 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) and urine.

Detection Range

0.78 ng/mL - 50 ng/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1301 6028 24574 1319 6108 24732 Standard deviation 53.34 373.74 1105.83 61.99 390.91 1335.53 CV (%) 4.1% 6.2% 4.5% 4.7% 6.4% 5.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1301 6028 24574 1319 6108 24732 Standard deviation 53.34 373.74 1105.83 61.99 390.91 1335.53 CV (%) 4.1% 6.2% 4.5% 4.7% 6.4% 5.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1301 6028 24574 1319 6108 24732 Mean (pg/ml) 1301 6028 24574 1319 6108 24732 Standard deviation 53.34 373.74 1105.83 61.99 390.91 1335.53 Standard deviation 53.34 373.74 1105.83 61.99 390.91 1335.53 CV (%) 4.1% 6.2% 4.5% 4.7% 6.4% 5.4% CV (%) 4.1% 6.2% 4.5% 4.7% 6.4% 5.4%

Sensitivity

<50 pg/mL

Function

Reversible hydration of carbon dioxide.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human CA3

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Carbonic anhydrase 3

Subcellular Location

Cytoplasm

Sample Data

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.058 0.151 0.213 0.335 0.625 0.949 1.507 2.384 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.058 0.151 0.213 0.335 0.625 0.949 1.507 2.384 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.058 0.151 0.213 0.335 0.625 0.949 1.507 2.384 O.D. 0.058 0.151 0.213 0.335 0.625 0.949 1.507 2.384

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.