Human CD55 ELISA Kit PicoKine®

Complement decay-accelerating factor, also known as CD55 or DAF, is a protein that, in humans, is encoded by the CD55 gene. It is mapped to 1q32.2. This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM) . Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 541 588 498 497 531 37.39 7.0% Sample 2 2056 2136 2100 2036 2082 38.83 1.8% Sample 3 8927 8943 9804 10335 9502 597.53 6.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 541 588 498 497 531 37.39 7.0% Sample 1 541 588 498 497 531 37.39 7.0% Sample 2 2056 2136 2100 2036 2082 38.83 1.8% Sample 2 2056 2136 2100 2036 2082 38.83 1.8% Sample 3 8927 8943 9804 10335 9502 597.53 6.2% Sample 3 8927 8943 9804 10335 9502 597.53 6.2%

Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body fluids and in extracellular matrix.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 541 2056 8927 588 2124 9804 Standard deviation 23.26 133.64 419.57 36.46 104.08 696.08 CV (%) 4.3% 6.5% 4.7% 6.2% 4.9% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 541 2056 8927 588 2124 9804 Standard deviation 23.26 133.64 419.57 36.46 104.08 696.08 CV (%) 4.3% 6.5% 4.7% 6.2% 4.9% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 541 2056 8927 588 2124 9804 Mean (pg/ml) 541 2056 8927 588 2124 9804 Standard deviation 23.26 133.64 419.57 36.46 104.08 696.08 Standard deviation 23.26 133.64 419.57 36.46 104.08 696.08 CV (%) 4.3% 6.5% 4.7% 6.2% 4.9% 7.1% CV (%) 4.3% 6.5% 4.7% 6.2% 4.9% 7.1%

This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. Inhibits complement activation by destabilizing and preventing the formation of C3 and C5 convertases, which prevents complement damage.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.03 0.085 0.138 0.229 0.423 0.806 1.391 2.271 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.03 0.085 0.138 0.229 0.423 0.806 1.391 2.271 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.03 0.085 0.138 0.229 0.423 0.806 1.391 2.271 O.D. 0.03 0.085 0.138 0.229 0.423 0.806 1.391 2.271

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.