Human CHRDL1 ELISA Kit PicoKine®

Chordin-like 1 is a protein that in humans is encoded by the CHRDL1 gene. It is mapped to Xq23. This gene encodes an antagonist of bone morphogenetic protein 4. The encoded protein may play a role in topographic retinotectal projection and in the regulation of retinal angiogenesis in response to hypoxia. Alternatively spliced transcript variants encoding different isoforms have been described.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 146 138 126 133 135 7.29 5.4% Sample 2 511 424 442 453 457 32.57 7.1% Sample 3 1796 1945 1901 2041 1920 88.03 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 146 138 126 133 135 7.29 5.4% Sample 1 146 138 126 133 135 7.29 5.4% Sample 2 511 424 442 453 457 32.57 7.1% Sample 2 511 424 442 453 457 32.57 7.1% Sample 3 1796 1945 1901 2041 1920 88.03 4.5% Sample 3 1796 1945 1901 2041 1920 88.03 4.5%

Expressed in the developing cornea and in the eye anterior segment in addition to the retina. Differentially expressed in the fetal brain. There is high expression in cerebellum and neocortex. Expressed in retinal pericytes.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 146 511 1796 201 618 2851 Standard deviation 7.59 27.08 100.58 13.07 41.41 196.72 CV (%) 5.2% 5.3% 5.6% 6.5% 6.7% 6.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 146 511 1796 201 618 2851 Standard deviation 7.59 27.08 100.58 13.07 41.41 196.72 CV (%) 5.2% 5.3% 5.6% 6.5% 6.7% 6.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 146 511 1796 201 618 2851 Mean (pg/ml) 146 511 1796 201 618 2851 Standard deviation 7.59 27.08 100.58 13.07 41.41 196.72 Standard deviation 7.59 27.08 100.58 13.07 41.41 196.72 CV (%) 5.2% 5.3% 5.6% 6.5% 6.7% 6.9% CV (%) 5.2% 5.3% 5.6% 6.5% 6.7% 6.9%

Antagonizes the function of BMP4 by binding to it and preventing its interaction with receptors. Alters the fate commitment of neural stem cells from gliogenesis to neurogenesis. Contributes to neuronal differentiation of neural stem cells in the brain by preventing the adoption of a glial fate. May play a crucial role in dorsoventral axis formation. May play a role in embryonic bone formation. May also play an important role in regulating retinal angiogenesis through modulation of BMP4 actions in endothelial cells. Plays a role during anterior segment eye development.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.034 0.098 0.15 0.303 0.486 0.951 1.628 2.289 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.034 0.098 0.15 0.303 0.486 0.951 1.628 2.289 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.034 0.098 0.15 0.303 0.486 0.951 1.628 2.289 O.D. 0.034 0.098 0.15 0.303 0.486 0.951 1.628 2.289

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.