Human Complement C1R ELISA Kit PicoKine®

Human Complement C1R ELISA Kit PicoKine® (96 Tests) . Quantitate Human C1R in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) and urine. Sensitivity: 0.1ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Complement C1r subcomponent is a protein involved in the complement system of the innate immune system. It is mapped to 12p13.31. In humans, C1r is encoded by the C1R gene. C1r along with C1q and C1s form the C1 complex, which is the first component of the serum complement system. C1r is an enzyme that activates C1s to its active form, by proteolytic cleavage.C1r has been shown to interact with C1s. C1r cleaves C1s to form the active form of C1s.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 2705 2824 2677 2767 2743 56.86 2.0% Sample 2 11224 12397 11914 11121 11664 521.59 4.4% Sample 3 42475 48901 43488 50585 46362 3451.40 7.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 2705 2824 2677 2767 2743 56.86 2.0% Sample 1 2705 2824 2677 2767 2743 56.86 2.0% Sample 2 11224 12397 11914 11121 11664 521.59 4.4% Sample 2 11224 12397 11914 11121 11664 521.59 4.4% Sample 3 42475 48901 43488 50585 46362 3451.40 7.4% Sample 3 42475 48901 43488 50585 46362 3451.40 7.4%

Synonyms

Complement C1r subcomponent; Complement component 1 subcomponent r; C1R

Gene Name

C1R

Gene ID

715

UniProt

P00736

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S18-D705

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Applications

ELISA

Field of Research

Complement, Immunology, Innate Immunity

Standard

100 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) and urine.

Detection Range

1.56 ng/mL - 100 ng/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2705 11224 42475 3058 11987 42819 Standard deviation 129.84 583.65 1953.85 186.54 707.23 2740.42 CV (%) 4.8% 5.2% 4.6% 6.1% 5.9% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2705 11224 42475 3058 11987 42819 Standard deviation 129.84 583.65 1953.85 186.54 707.23 2740.42 CV (%) 4.8% 5.2% 4.6% 6.1% 5.9% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 2705 11224 42475 3058 11987 42819 Mean (pg/ml) 2705 11224 42475 3058 11987 42819 Standard deviation 129.84 583.65 1953.85 186.54 707.23 2740.42 Standard deviation 129.84 583.65 1953.85 186.54 707.23 2740.42 CV (%) 4.8% 5.2% 4.6% 6.1% 5.9% 6.4% CV (%) 4.8% 5.2% 4.6% 6.1% 5.9% 6.4%

Sensitivity

<0.1 ng/mL

Function

C1r B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human C1R

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Complement C1r

Subcellular Location

Secreted

Sample Data

Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.064 0.147 0.181 0.269 0.424 0.712 1.286 2.23 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.064 0.147 0.181 0.269 0.424 0.712 1.286 2.23 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.064 0.147 0.181 0.269 0.424 0.712 1.286 2.23 O.D. 0.064 0.147 0.181 0.269 0.424 0.712 1.286 2.23

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.