Human CA4/Carbonic anhydrase 4 ELISA Kit PicoKine®

Carbonic anhydrase 4 is an enzyme that in humans is encoded by the CA4 gene. It is mapped to 17q23.1. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This gene encodes a glycosylphosphatidyl-inositolanchored membrane isozyme expressed on the luminal surfaces of pulmonary (and certain other) capillaries and proximal renal tubules. Its exact function is not known; however, it may have a role in inherited renal abnormalities of bicarbonate transport.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 553 594 614 530 572 33.05 5.7% Sample 2 2529 2953 2486 2631 2649 182.82 6.9% Sample 3 8599 7975 9228 7943 8436 526.64 6.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 553 594 614 530 572 33.05 5.7% Sample 1 553 594 614 530 572 33.05 5.7% Sample 2 2529 2953 2486 2631 2649 182.82 6.9% Sample 2 2529 2953 2486 2631 2649 182.82 6.9% Sample 3 8599 7975 9228 7943 8436 526.64 6.2% Sample 3 8599 7975 9228 7943 8436 526.64 6.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 553 2529 8599 614 2686 9228 Standard deviation 42.03 123.92 481.54 41.75 139.67 544.45 CV (%) 7.6% 4.9% 5.6% 6.8% 5.2% 5.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 553 2529 8599 614 2686 9228 Standard deviation 42.03 123.92 481.54 41.75 139.67 544.45 CV (%) 7.6% 4.9% 5.6% 6.8% 5.2% 5.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 553 2529 8599 614 2686 9228 Mean (pg/ml) 553 2529 8599 614 2686 9228 Standard deviation 42.03 123.92 481.54 41.75 139.67 544.45 Standard deviation 42.03 123.92 481.54 41.75 139.67 544.45 CV (%) 7.6% 4.9% 5.6% 6.8% 5.2% 5.9% CV (%) 7.6% 4.9% 5.6% 6.8% 5.2% 5.9%

Reversible hydration of carbon dioxide. May stimulate the sodium/bicarbonate transporter activity of SLC4A4 that acts in pH homeostasis. It is essential for acid overload removal from the retina and retina epithelium, and acid release in the choriocapillaris in the choroid.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.018 0.098 0.197 0.394 0.769 1.23 1.869 2.263 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.018 0.098 0.197 0.394 0.769 1.23 1.869 2.263 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.018 0.098 0.197 0.394 0.769 1.23 1.869 2.263 O.D. 0.018 0.098 0.197 0.394 0.769 1.23 1.869 2.263

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.