Human CA12/Carbonic anhydrase 12 ELISA Kit PicoKine®

Carbonic anhydrase 12 is an enzyme that in humans is encoded by the CA12 gene. It is mapped to 15q22.2. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Three transcript variants encoding different isoforms have been identified for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 57 53 57 58 56 1.92 3.4% Sample 2 217 213 213 196 209 8.10 3.8% Sample 3 886 841 804 777 827 827 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 57 53 57 58 56 1.92 3.4% Sample 1 57 53 57 58 56 1.92 3.4% Sample 2 217 213 213 196 209 8.10 3.8% Sample 2 217 213 213 196 209 8.10 3.8% Sample 3 886 841 804 777 827 827 4.9% Sample 3 886 841 804 777 827 827 4.9%

Highly expressed in colon, kidney, prostate, intestine and activated lymphocytes. Expressed at much higher levels in the renal cell cancers than in surrounding normal kidney tissue. Moderately expressed in pancreas, ovary and testis.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 57 217 886 60 231 927 Standard deviation 3.19 8.90 46.96 2.88 14.32 50.99 CV (%) 5.6% 4.1% 5.3% 4.8% 6.2% 5.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 57 217 886 60 231 927 Standard deviation 3.19 8.90 46.96 2.88 14.32 50.99 CV (%) 5.6% 4.1% 5.3% 4.8% 6.2% 5.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 57 217 886 60 231 927 Mean (pg/ml) 57 217 886 60 231 927 Standard deviation 3.19 8.90 46.96 2.88 14.32 50.99 Standard deviation 3.19 8.90 46.96 2.88 14.32 50.99 CV (%) 5.6% 4.1% 5.3% 4.8% 6.2% 5.5% CV (%) 5.6% 4.1% 5.3% 4.8% 6.2% 5.5%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.041 0.143 0.223 0.354 0.589 0.96 1.802 2.762 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.041 0.143 0.223 0.354 0.589 0.96 1.802 2.762 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.041 0.143 0.223 0.354 0.589 0.96 1.802 2.762 O.D. 0.041 0.143 0.223 0.354 0.589 0.96 1.802 2.762

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.