Rat SMOC1 ELISA Kit PicoKine®

Rat SMOC1 ELISA Kit PicoKine® (96 Tests) . Quantitate Rat Smoc1 in cell culture supernatants, serum and plasma (heparin) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

This gene is mapped to 6q24. Human homolog binds calcium and may play a role in the extracellular matrix.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 159 162 177 168 167 5.49 3.3% Sample 2 688 757 693 684 706 14.11 2.0% Sample 3 1452 1596 1441 1365 1464 83.42 5.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 159 162 177 168 167 5.49 3.3% Sample 1 159 162 177 168 167 5.49 3.3% Sample 2 688 757 693 684 706 14.11 2.0% Sample 2 688 757 693 684 706 14.11 2.0% Sample 3 1452 1596 1441 1365 1464 83.42 5.7% Sample 3 1452 1596 1441 1365 1464 83.42 5.7%

Synonyms

SPARC-related modular calcium binding protein 1; SPARC-related modular calcium-binding 1; Smoc1; smoc1

Gene Name

Smoc1

Gene ID

314280

UniProt

Q6IE50

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: H26-V452

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 159 688 1452 168 675 1526 Standard deviation 7.63 39.22 66.79 8.57 39.83 97.66 CV (%) 4.8% 5.7% 4.6% 5.1% 5.9% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 159 688 1452 168 675 1526 Standard deviation 7.63 39.22 66.79 8.57 39.83 97.66 CV (%) 4.8% 5.7% 4.6% 5.1% 5.9% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 159 688 1452 168 675 1526 Mean (pg/ml) 159 688 1452 168 675 1526 Standard deviation 7.63 39.22 66.79 8.57 39.83 97.66 Standard deviation 7.63 39.22 66.79 8.57 39.83 97.66 CV (%) 4.8% 5.7% 4.6% 5.1% 5.9% 6.4% CV (%) 4.8% 5.7% 4.6% 5.1% 5.9% 6.4%

Sensitivity

<10 pg/mL

Function

Lacks conserved residue (s) required for the propagation of feature annotation.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant rat SMOC1

Anticoagulant

Heparin

Gene Name Synonym

SPARC related modular calcium binding 1

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.044 0.0861 0.126 0.206 0.36 0.608 1.1 1.806 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.044 0.0861 0.126 0.206 0.36 0.608 1.1 1.806 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.044 0.0861 0.126 0.206 0.36 0.608 1.1 1.806 O.D. 0.044 0.0861 0.126 0.206 0.36 0.608 1.1 1.806

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.