Mouse C1R ELISA Kit PicoKine®

Mouse C1R ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse C1ra in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) and urine. Sensitivity: 0.1ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Complement C1r subcomponent?is a?protein?involved in the?complement system?of the?innate immune system.?In humans, C1r is encoded by the?C1R?gene. It is mapped to 6; 6 F2 and 6 F2; 6. C1r has been shown to interact with C1s. C1r cleaves C1s to form the active form of C1s.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1004 1190 1038 1174 1101 81.58 7.4% Sample 2 3663 4171 3599 3605 3759 238.89 6.3% Sample 3 23406 24315 20399 23979 23024 1550.42 6.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1004 1190 1038 1174 1101 81.58 7.4% Sample 1 1004 1190 1038 1174 1101 81.58 7.4% Sample 2 3663 4171 3599 3605 3759 238.89 6.3% Sample 2 3663 4171 3599 3605 3759 238.89 6.3% Sample 3 23406 24315 20399 23979 23024 1550.42 6.7% Sample 3 23406 24315 20399 23979 23024 1550.42 6.7%

Synonyms

Complement C1r-A subcomponent; Complement component 1 subcomponent r-A; C1ra; C1r; Complement C1r-B subcomponent; Complement component 1 subcomponent r-B; C1rb

Gene Name

C1ra

Gene ID

50909

UniProt

Q8CG16; Q8CFG9

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S18-D705

Capture Antibody

A monoclonal antibody from rat

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Tissue Specificity

Specifically expressed in male reproductive tissues.

Applications

ELISA

Standard

40 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) and urine.

Detection Range

0.625 ng/mL - 40 ng/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1004 3663 23406 1174 4272 24979 Standard deviation 47.19 197.80 1451.17 79.83 196.51 1448.78 CV (%) 4.7% 5.4% 6.2% 6.8% 4.6% 5.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1004 3663 23406 1174 4272 24979 Standard deviation 47.19 197.80 1451.17 79.83 196.51 1448.78 CV (%) 4.7% 5.4% 6.2% 6.8% 4.6% 5.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1004 3663 23406 1174 4272 24979 Mean (pg/ml) 1004 3663 23406 1174 4272 24979 Standard deviation 47.19 197.80 1451.17 79.83 196.51 1448.78 Standard deviation 47.19 197.80 1451.17 79.83 196.51 1448.78 CV (%) 4.7% 5.4% 6.2% 6.8% 4.6% 5.8% CV (%) 4.7% 5.4% 6.2% 6.8% 4.6% 5.8%

Sensitivity

<0.1 ng/mL

Function

C1r B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse C1R

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Complement component 1, r subcomponent A; complement component 1, r subcomponent B

Subcellular Location

Extracellular space

Sample Data

Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.012 0.056 0.089 0.181 0.365 0.578 1.091 1.805 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.012 0.056 0.089 0.181 0.365 0.578 1.091 1.805 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.012 0.056 0.089 0.181 0.365 0.578 1.091 1.805 O.D. 0.012 0.056 0.089 0.181 0.365 0.578 1.091 1.805

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.