Human CREG1 ELISA Kit PicoKine®

This gene is mapped to 1q24.2. The adenovirus E1A protein both activates and represses gene expression to promote cellular proliferation and inhibit differentiation. The protein encoded by this gene antagonizes transcriptional activation and cellular transformation by E1A. This protein shares limited sequence similarity with E1A and binds both the general transcription factor TBP and the tumor suppressor pRb in vitro. This gene may contribute to the transcriptional control of cell growth and differentiation.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 441 427 436 475 444 18.17 4.0% Sample 2 1500 1602 1545 1419 1516 66.89 4.4% Sample 3 8633 8533 7903 8527 8399 289.44 3.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 441 427 436 475 444 18.17 4.0% Sample 1 441 427 436 475 444 18.17 4.0% Sample 2 1500 1602 1545 1419 1516 66.89 4.4% Sample 2 1500 1602 1545 1419 1516 66.89 4.4% Sample 3 8633 8533 7903 8527 8399 289.44 3.4% Sample 3 8633 8533 7903 8527 8399 289.44 3.4%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 441 1500 8633 514 2089 9813 Standard deviation 21.61 79.50 578.41 32.90 100.27 578.97 CV (%) 4.9% 5.3% 6.7% 6.4% 4.8% 5.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 441 1500 8633 514 2089 9813 Standard deviation 21.61 79.50 578.41 32.90 100.27 578.97 CV (%) 4.9% 5.3% 6.7% 6.4% 4.8% 5.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 441 1500 8633 514 2089 9813 Mean (pg/ml) 441 1500 8633 514 2089 9813 Standard deviation 21.61 79.50 578.41 32.90 100.27 578.97 Standard deviation 21.61 79.50 578.41 32.90 100.27 578.97 CV (%) 4.9% 5.3% 6.7% 6.4% 4.8% 5.9% CV (%) 4.9% 5.3% 6.7% 6.4% 4.8% 5.9%

May contribute to the transcriptional control of cell growth and differentiation. Antagonizes transcriptional activation and cellular transformation by the adenovirus E1A protein. The transcriptional control activity of cell growth requires interaction with IGF2R.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.021 0.183 0.23 0.386 0.596 0.991 1.661 2.095 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.021 0.183 0.23 0.386 0.596 0.991 1.661 2.095 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.021 0.183 0.23 0.386 0.596 0.991 1.661 2.095 O.D. 0.021 0.183 0.23 0.386 0.596 0.991 1.661 2.095

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.