Human CA1/Carbonic anhydrase 1 ELISA Kit PicoKine®

Human CA1 ELISA Kit PicoKine® (96 Tests) . Quantitate Human CA1 in cell culture supernatants, cell lysates, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Carbonic anhydrase 1 is an enzyme that in humans is encoded by the CA1 gene. It is a member of the Carbonic anhydrase. The CA1 gene is mapped to 8q22. CAI has got about 260 amino acids. This protein is highly expressed in erythrocytes. As catalysts of the reversible hydration of carbon dioxide, CAI participates in a variety of biologic processes like respiration, calcification, acid-base balance etc.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 452 501 491 473 479 18.65 3.8% Sample 2 2590 2841 2800 2781 2753 96.57 3.5% Sample 3 9786 9681 9685 8581 9433 493.84 5.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 452 501 491 473 479 18.65 3.8% Sample 1 452 501 491 473 479 18.65 3.8% Sample 2 2590 2841 2800 2781 2753 96.57 3.5% Sample 2 2590 2841 2800 2781 2753 96.57 3.5% Sample 3 9786 9681 9685 8581 9433 493.84 5.2% Sample 3 9786 9681 9685 8581 9433 493.84 5.2%

Synonyms

Carbonic anhydrase 1; Carbonate dehydratase I; Carbonic anhydrase B; CAB; Carbonic anhydrase I; CA-I; CA1

Gene Name

CA1

Gene ID

759

UniProt

P00915

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A2-F261

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Applications

ELISA

Field of Research

Angiogenesis, Cancer, Cell Adhesion, Cell Adhesion Proteins, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Membrane Proteins, Neuroscience, Signal Transduction

Standard

20 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum, plasma (heparin, EDTA, citrate) and urine.

Detection Range

312 pg/mL - 20,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 452 2590 9786 584 2656 9986 Standard deviation 18.98 157.99 636.09 31.54 114.21 559.22 CV (%) 4.2% 6.1% 6.5% 5.4% 4.3% 5.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 452 2590 9786 584 2656 9986 Standard deviation 18.98 157.99 636.09 31.54 114.21 559.22 CV (%) 4.2% 6.1% 6.5% 5.4% 4.3% 5.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 452 2590 9786 584 2656 9986 Mean (pg/ml) 452 2590 9786 584 2656 9986 Standard deviation 18.98 157.99 636.09 31.54 114.21 559.22 Standard deviation 18.98 157.99 636.09 31.54 114.21 559.22 CV (%) 4.2% 6.1% 6.5% 5.4% 4.3% 5.6% CV (%) 4.2% 6.1% 6.5% 5.4% 4.3% 5.6%

Sensitivity

<50 pg/mL

Function

Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human CA1

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Carbonic anhydrase 1

Subcellular Location

Cytoplasm

Sample Data

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.006 0.074 0.146 0.296 0.509 0.948 1.353 1.903 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.006 0.074 0.146 0.296 0.509 0.948 1.353 1.903 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.006 0.074 0.146 0.296 0.509 0.948 1.353 1.903 O.D. 0.006 0.074 0.146 0.296 0.509 0.948 1.353 1.903

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.