Human CLEC11A ELISA Kit PicoKine®

This gene encodes a member of the C-type lectin superfamily. The encoded protein is a secreted sulfated glycoprotein and functions as a growth factor for primitive hematopoietic progenitor cells. An alternative splice variant has been described but its biological nature has not been determined. It is mapped to 19q13.33.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 270 284 305 296 288 13.14 4.5% Sample 2 1292 1291 1147 1271 1250 60.19 4.8% Sample 3 4732 4272 4489 4168 4415 216.46 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 270 284 305 296 288 13.14 4.5% Sample 1 270 284 305 296 288 13.14 4.5% Sample 2 1292 1291 1147 1271 1250 60.19 4.8% Sample 2 1292 1291 1147 1271 1250 60.19 4.8% Sample 3 4732 4272 4489 4168 4415 216.46 4.9% Sample 3 4732 4272 4489 4168 4415 216.46 4.9%

C-type lectin domain family 11 member A; C-type lectin superfamily member 3; Lymphocyte secreted C-type lectin; Osteolectin; Stem cell growth factor; p47; CLEC11A; CLECSF3; LSLCL; SCGF

Expressed in skeletal tissues including bone marrow, chondrocytes, primary ossification center-associated cells, the perichondrium and periosteum. Lower levels of expression were detected in spleen, thymus, appendix and fetal liver.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 270 1292 4732 284 1347 4986 Standard deviation 19.44 81.40 212.94 15.05 86.21 209.41 CV (%) 7.2% 6.3% 4.5% 5.3% 6.4% 4.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 270 1292 4732 284 1347 4986 Standard deviation 19.44 81.40 212.94 15.05 86.21 209.41 CV (%) 7.2% 6.3% 4.5% 5.3% 6.4% 4.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 270 1292 4732 284 1347 4986 Mean (pg/ml) 270 1292 4732 284 1347 4986 Standard deviation 19.44 81.40 212.94 15.05 86.21 209.41 Standard deviation 19.44 81.40 212.94 15.05 86.21 209.41 CV (%) 7.2% 6.3% 4.5% 5.3% 6.4% 4.2% CV (%) 7.2% 6.3% 4.5% 5.3% 6.4% 4.2%

Promotes osteogenesis by stimulating the differentiation of mesenchymal progenitors into mature osteoblasts. Important for repair and maintenance of adult bone.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.042 0.077 0.108 0.167 0.301 0.534 1.0 1.745 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.042 0.077 0.108 0.167 0.301 0.534 1.0 1.745 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.042 0.077 0.108 0.167 0.301 0.534 1.0 1.745 O.D. 0.042 0.077 0.108 0.167 0.301 0.534 1.0 1.745

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.