Human CA6/Carbonic anhydrase 6 ELISA Kit PicoKine®

Human CA6 ELISA Kit PicoKine® (96 Tests) . Quantitate Human CA6 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

This gene is mapped to 1p36.23. The protein encoded by this gene is one of several isozymes of carbonic anhydrase. This protein is found only in salivary glands and saliva and protein may play a role in the reversible hydratation of carbon dioxide though its function in saliva is unknown.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 228 250 230 225 233 9.83 4.2% Sample 2 1372 1388 1260 1370 1347 50.99 3.7% Sample 3 5130 4907 4480 4360 4719 312.35 6.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 228 250 230 225 233 9.83 4.2% Sample 1 228 250 230 225 233 9.83 4.2% Sample 2 1372 1388 1260 1370 1347 50.99 3.7% Sample 2 1372 1388 1260 1370 1347 50.99 3.7% Sample 3 5130 4907 4480 4360 4719 312.35 6.6% Sample 3 5130 4907 4480 4360 4719 312.35 6.6%

Synonyms

Carbonic anhydrase 6; Carbonate dehydratase VI; Carbonic anhydrase VI; CA-VI; Salivary carbonic anhydrase; Secreted carbonic anhydrase

Gene Name

CA6

Gene ID

765

UniProt

P23280

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q18-N308

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Tissue Specificity

Major constituent of saliva.

Applications

ELISA

Field of Research

Cell Biology

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 228 1372 5130 249 1437 5217 Standard deviation 14.14 72.72 241.11 15.19 106.34 266.07 CV (%) 6.2% 5.3% 4.7% 6.1% 7.4% 5.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 228 1372 5130 249 1437 5217 Standard deviation 14.14 72.72 241.11 15.19 106.34 266.07 CV (%) 6.2% 5.3% 4.7% 6.1% 7.4% 5.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 228 1372 5130 249 1437 5217 Mean (pg/ml) 228 1372 5130 249 1437 5217 Standard deviation 14.14 72.72 241.11 15.19 106.34 266.07 Standard deviation 14.14 72.72 241.11 15.19 106.34 266.07 CV (%) 6.2% 5.3% 4.7% 6.1% 7.4% 5.1% CV (%) 6.2% 5.3% 4.7% 6.1% 7.4% 5.1%

Sensitivity

<50 pg/mL

Function

Reversible hydration of carbon dioxide. Its role in saliva is unknown.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human CA6

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Carbonic anhydrase 6

Subcellular Location

Secreted

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.055 0.101 0.152 0.242 0.417 0.743 1.221 1.826 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.055 0.101 0.152 0.242 0.417 0.743 1.221 1.826 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.055 0.101 0.152 0.242 0.417 0.743 1.221 1.826 O.D. 0.055 0.101 0.152 0.242 0.417 0.743 1.221 1.826

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.