Human Nectin-3/CD113 ELISA Kit PicoKine®

Human Nectin-3/CD113 ELISA Kit PicoKine® (96 Tests) . Quantitate Human NECTIN3 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Poliovirus receptor-related 3 (PVRL3), also known as nectin-3 and CD113, is a human protein of the immunoglobulin superfamily which forms part of adherens junctions. It is mapped to 19q13.32. This gene encodes a member of the nectin family of proteins, which function as adhesion molecules at adherens junctions. This family member interacts with other nectin-like proteins and with afadin, a filamentous actin-binding protein involved in the regulation of ional motility, cell proliferation and survival. This gene plays a role in ocular development involving the ciliary body. Mutations in this gene are believed to result in congenital ocular defects. Alternative splicing results in multiple transcript variants.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 159 143 139 152 148 7.79 5.2% Sample 2 873 812 883 939 876 45.05 5.1% Sample 3 3809 3570 3780 3280 3609 211.54 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 159 143 139 152 148 7.79 5.2% Sample 1 159 143 139 152 148 7.79 5.2% Sample 2 873 812 883 939 876 45.05 5.1% Sample 2 873 812 883 939 876 45.05 5.1% Sample 3 3809 3570 3780 3280 3609 211.54 5.8% Sample 3 3809 3570 3780 3280 3609 211.54 5.8%

Synonyms

Nectin-3; CDw113; Nectin cell adhesion molecule 3; Poliovirus receptor-related protein 3; CD113; NECTIN3; PRR3; PVRL3

Gene Name

NECTIN3

Gene ID

25945

UniProt

Q9NQS3

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: G58-T404

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Tissue Specificity

Predominantly expressed in testis and placenta as well as in many cell lines, including epithelial cell lines.

Applications

ELISA

Field of Research

Host-Virus Interaction, Immunoglobulins, Immunology, Interspecies Interaction, Microbiology, Receptors

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

125 pg/mL - 8,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 159 873 3809 239 939 3980 Standard deviation 10.65 45.40 217.11 15.06 50.71 163.18 CV (%) 6.7% 5.2% 5.7% 6.3% 5.4% 4.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 159 873 3809 239 939 3980 Standard deviation 10.65 45.40 217.11 15.06 50.71 163.18 CV (%) 6.7% 5.2% 5.7% 6.3% 5.4% 4.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 159 873 3809 239 939 3980 Mean (pg/ml) 159 873 3809 239 939 3980 Standard deviation 10.65 45.40 217.11 15.06 50.71 163.18 Standard deviation 10.65 45.40 217.11 15.06 50.71 163.18 CV (%) 6.7% 5.2% 5.7% 6.3% 5.4% 4.1% CV (%) 6.7% 5.2% 5.7% 6.3% 5.4% 4.1%

Sensitivity

<20 pg/mL

Function

Plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with NECTIN2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human Nectin-3/CD113

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Nectin cell adhesion molecule 3

Subcellular Location

Cell membrane, Single-pass membrane protein, Postsynaptic cell membrane

Sample Data

Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.035 0.137 0.228 0.42 0.774 1.137 1.762 2.147 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.035 0.137 0.228 0.42 0.774 1.137 1.762 2.147 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 Concentration (pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.035 0.137 0.228 0.42 0.774 1.137 1.762 2.147 O.D. 0.035 0.137 0.228 0.42 0.774 1.137 1.762 2.147

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.