Human LY9 ELISA Kit PicoKine®

T-lymphocyte surface antigen Ly-9 is a protein that in humans is encoded by the LY9 gene. This gene is mapped to 17q21.31. LY9 has also recently been designated CD229. LY9 belongs to the SLAM family of immunomodulatory receptors and interacts with the adaptor molecule SAP.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 92 93 98 92 93 2.48 2.6% Sample 2 698 622 636 644 650 28.80 4.4% Sample 3 2164 1927 1955 2266 2078 142.01 6.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 92 93 98 92 93 2.48 2.6% Sample 1 92 93 98 92 93 2.48 2.6% Sample 2 698 622 636 644 650 28.80 4.4% Sample 2 698 622 636 644 650 28.80 4.4% Sample 3 2164 1927 1955 2266 2078 142.01 6.8% Sample 3 2164 1927 1955 2266 2078 142.01 6.8%

T-lymphocyte surface antigen Ly-9; Cell surface molecule Ly-9; Lymphocyte antigen 9; SLAM family member 3; SLAMF3; Signaling lymphocytic activation molecule 3; CD229; LY9; CDABP0070

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 92 698 2164 108 736 2366 Standard deviation 5.43 30.01 110.36 7.13 42.69 108.84 CV (%) 5.9% 4.3% 5.1% 6.6% 5.8% 4.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 92 698 2164 108 736 2366 Standard deviation 5.43 30.01 110.36 7.13 42.69 108.84 CV (%) 5.9% 4.3% 5.1% 6.6% 5.8% 4.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 92 698 2164 108 736 2366 Mean (pg/ml) 92 698 2164 108 736 2366 Standard deviation 5.43 30.01 110.36 7.13 42.69 108.84 Standard deviation 5.43 30.01 110.36 7.13 42.69 108.84 CV (%) 5.9% 4.3% 5.1% 6.6% 5.8% 4.6% CV (%) 5.9% 4.3% 5.1% 6.6% 5.8% 4.6%

Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. May participate in adhesion reactions between T lymphocytes and accessory cells by homophilic interaction. Promotes T-cell differentiation into a helper T-cell Th17 phenotype leading to increased IL-17 secretion; the costimulatory activity requires SH2D1A (PubMed:22184727) . Promotes recruitment of RORC to the IL-17 promoter (PubMed:22989874) . May be involved in the maintenance of peripheral cell tolerance by serving as a negative regulator of the immune response. May disable autoantibody responses and inhibit IFN-gamma secretion by CD4+ T-cells. May negatively regulate the size of thymic innate CD8+ T-cells and the development of invariant natural killer T (iNKT) cells (By similarity) .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.006 0.079 0.117 0.214 0.403 0.701 1.218 2.037 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.006 0.079 0.117 0.214 0.403 0.701 1.218 2.037 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.006 0.079 0.117 0.214 0.403 0.701 1.218 2.037 O.D. 0.006 0.079 0.117 0.214 0.403 0.701 1.218 2.037

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.