Human CFHR5 ELISA Kit PicoKine®

Complement factor H-related protein 5 is a protein that in humans is encoded by the CFHR5 gene. It is mapped to 1q31.3. This gene is a member of a small complement factor H (CFH) gene cluster on chromosome 1. Each member of this gene family contains multiple short consensus repeats (SCRs) typical of regulators of complement activation. The protein encoded by this gene has nine SCRs with the first two repeats having heparin binding properties, a region within repeats 5-7 having heparin binding and C reactive protein binding properties, and the C-terminal repeats being similar to a complement component 3 b (C3b) binding domain. This protein co-localizes with C3, binds C3b in a dose-dependent manner, and is recruited to tissues damaged by C-reactive protein. Allelic variations in this gene have been associated, but not causally linked, with two different forms of kidney disease: membranoproliferative glomerulonephritis type II (MPGNII) and hemolytic uraemic syndrome (HUS) .

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 230 223 199 303 239 8.69 3.6% Sample 2 321 623 589 427 490 38.51 7.9% Sample 3 2847 4318 3088 2545 3200 167.97 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 230 223 199 303 239 8.69 3.6% Sample 1 230 223 199 303 239 8.69 3.6% Sample 2 321 623 589 427 490 38.51 7.9% Sample 2 321 623 589 427 490 38.51 7.9% Sample 3 2847 4318 3088 2545 3200 167.97 5.3% Sample 3 2847 4318 3088 2545 3200 167.97 5.3%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 190 432 2544 294 510 4693 Standard deviation 12.24 18.14 170.19 12.94 36.77 334.61 CV (%) 6.4% 4.2% 6.7% 4.4% 7.2% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 190 432 2544 294 510 4693 Standard deviation 12.24 18.14 170.19 12.94 36.77 334.61 CV (%) 6.4% 4.2% 6.7% 4.4% 7.2% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 190 432 2544 294 510 4693 Mean (pg/ml) 190 432 2544 294 510 4693 Standard deviation 12.24 18.14 170.19 12.94 36.77 334.61 Standard deviation 12.24 18.14 170.19 12.94 36.77 334.61 CV (%) 6.4% 4.2% 6.7% 4.4% 7.2% 7.1% CV (%) 6.4% 4.2% 6.7% 4.4% 7.2% 7.1%

Involved in complement regulation. The dimerized forms have avidity for tissue-bound complement fragments and efficiently compete with the physiological complement inhibitor CFH.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.006 0.079 0.117 0.214 0.403 0.701 1.218 2.037 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.006 0.079 0.117 0.214 0.403 0.701 1.218 2.037 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.006 0.079 0.117 0.214 0.403 0.701 1.218 2.037 O.D. 0.006 0.079 0.117 0.214 0.403 0.701 1.218 2.037

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.