Human C1S ELISA Kit PicoKine®

Complement component 1s is a protein involved in the complement system. It is mapped to 12p13.31. This gene encodes a serine protease, which is a major constituent of the human complement subcomponent C1. C1s associates with two other complement components C1r and C1q in order to yield the first component of the serum complement system. Defects in this gene are the cause of selective C1s deficiency.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1236 1246 1393 1346 1305 66.45 5.0% Sample 2 4483 4300 3867 4110 4190 228.40 5.4% Sample 3 22854 23761 20481 22796 22473 1212.07 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1236 1246 1393 1346 1305 66.45 5.0% Sample 1 1236 1246 1393 1346 1305 66.45 5.0% Sample 2 4483 4300 3867 4110 4190 228.40 5.4% Sample 2 4483 4300 3867 4110 4190 228.40 5.4% Sample 3 22854 23761 20481 22796 22473 1212.07 5.3% Sample 3 22854 23761 20481 22796 22473 1212.07 5.3%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1236 4483 22854 1393 4567 22796 Standard deviation 76.63 300.36 1028.43 82.19 324.26 980.23 CV (%) 6.2% 6.7% 4.5% 5.9% 7.1% 4.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1236 4483 22854 1393 4567 22796 Standard deviation 76.63 300.36 1028.43 82.19 324.26 980.23 CV (%) 6.2% 6.7% 4.5% 5.9% 7.1% 4.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1236 4483 22854 1393 4567 22796 Mean (pg/ml) 1236 4483 22854 1393 4567 22796 Standard deviation 76.63 300.36 1028.43 82.19 324.26 980.23 Standard deviation 76.63 300.36 1028.43 82.19 324.26 980.23 CV (%) 6.2% 6.7% 4.5% 5.9% 7.1% 4.3% CV (%) 6.2% 6.7% 4.5% 5.9% 7.1% 4.3%

C1s B chain is a serine protease that combines with C1q and C1r to form C1, the first component of the classical pathway of the complement system. C1r activates C1s so that it can, in turn, activate C2 and C4.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.005 0.052 0.078 0.154 0.294 0.573 1.091 1.86 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.005 0.052 0.078 0.154 0.294 0.573 1.091 1.86 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.005 0.052 0.078 0.154 0.294 0.573 1.091 1.86 O.D. 0.005 0.052 0.078 0.154 0.294 0.573 1.091 1.86

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.