Human FCRL5 ELISA Kit PicoKine®

Fc receptor-like protein 5 is a protein that in humans is encoded by the FCRL5 gene. It is mapped to 1q23.1. This gene encodes a member of the immunoglobulin receptor superfamily and the Fc-receptor like family. This gene and several other Fc receptor-like gene members are clustered on the long arm of chromosome 1. The encoded protein is a single-pass type I membrane protein and contains 8 immunoglobulin-like C2-type domains. This gene is implicated in B cell development and lymphomagenesis. Alternatively spliced transcript variants encoding different isoforms have been identified.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1090 1154 1262 1129 1158 63.82 5.5% Sample 2 6877 7371 6834 7977 7264 462.19 6.3% Sample 3 21168 22254 20007 18723 20538 1315.08 6.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1090 1154 1262 1129 1158 63.82 5.5% Sample 1 1090 1154 1262 1129 1158 63.82 5.5% Sample 2 6877 7371 6834 7977 7264 462.19 6.3% Sample 2 6877 7371 6834 7977 7264 462.19 6.3% Sample 3 21168 22254 20007 18723 20538 1315.08 6.4% Sample 3 21168 22254 20007 18723 20538 1315.08 6.4%

FcR-like protein 5; FcRL5; BXMAS1; Fc receptor homolog 5; FcRH5; Immune receptor translocation-associated protein 2; CD307e; FCRH5; IRTA2; UNQ503/PRO820

Expressed in marginal zone B-cells, immunoblasts, tonsillar germinal center centrocytes and in the intraepithelial and interfollicular regions of the tonsil. Expressed in many lymphoma cell lines and on hairy cell leukemia cells. Isoform 1, isoform 3, isoform 4 and isoform 5 are detected in lymph node, spleen, bone marrow, and small intestine with preponderance of isoform 3. Expressed in mature and memory B-cells and down-regulated in germinal center cells.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1090 6877 21168 1262 6894 22254 Standard deviation 56.68 433.25 1016.06 61.84 510.16 1179.46 CV (%) 5.2% 6.3% 4.8% 4.9% 7.4% 5.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1090 6877 21168 1262 6894 22254 Standard deviation 56.68 433.25 1016.06 61.84 510.16 1179.46 CV (%) 5.2% 6.3% 4.8% 4.9% 7.4% 5.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1090 6877 21168 1262 6894 22254 Mean (pg/ml) 1090 6877 21168 1262 6894 22254 Standard deviation 56.68 433.25 1016.06 61.84 510.16 1179.46 Standard deviation 56.68 433.25 1016.06 61.84 510.16 1179.46 CV (%) 5.2% 6.3% 4.8% 4.9% 7.4% 5.3% CV (%) 5.2% 6.3% 4.8% 4.9% 7.4% 5.3%

May be involved in B-cell development and differentiation in peripheral lymphoid organs and may be useful markers of B-cell stages. May have an immunoregulatory role in marginal zone B-cells.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.058 0.12 0.164 0.321 0.52 0.842 1.348 2.081 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.058 0.12 0.164 0.321 0.52 0.842 1.348 2.081 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.058 0.12 0.164 0.321 0.52 0.842 1.348 2.081 O.D. 0.058 0.12 0.164 0.321 0.52 0.842 1.348 2.081

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.