Rat VE-Cadherin Cdh5 ELISA Kit PicoKine®

Cadherin 5, type 2 or VE-cadherin also known as CD144, is a type of cadherin. It is encoded by the human gene CDH5. This gene is mapped to 19p14. VE-cadherin is a classical cadherin from the cadherin superfamily and the gene is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. The encoded protein is a calcium-dependent cell–cell adhesion glycoprotein composed of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 399 410 443 467 429 26.92 6.2% Sample 2 1935 2194 2013 2122 2066 99.36 4.8% Sample 3 8758 8677 8447 8601 8620 114.65 1.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 399 410 443 467 429 26.92 6.2% Sample 1 399 410 443 467 429 26.92 6.2% Sample 2 1935 2194 2013 2122 2066 99.36 4.8% Sample 2 1935 2194 2013 2122 2066 99.36 4.8% Sample 3 8758 8677 8447 8601 8620 114.65 1.3% Sample 3 8758 8677 8447 8601 8620 114.65 1.3%

Atherosclerosis, Cadherins, Cancer, Cardiovascular, Cell Adhesion, Cell Adhesion Molecules, Cytoskeleton/ECM, Invasion/Microenvironment, Signal Transduction, Vascular Inflammation, Vasculature

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 399 1935 8758 443 2194 8947 Standard deviation 21.95 123.84 507.96 27.47 112.11 653.13 CV (%) 5.5% 6.4% 5.8% 6.2% 5.1% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 399 1935 8758 443 2194 8947 Standard deviation 21.95 123.84 507.96 27.47 112.11 653.13 CV (%) 5.5% 6.4% 5.8% 6.2% 5.1% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 399 1935 8758 443 2194 8947 Mean (pg/ml) 399 1935 8758 443 2194 8947 Standard deviation 21.95 123.84 507.96 27.47 112.11 653.13 Standard deviation 21.95 123.84 507.96 27.47 112.11 653.13 CV (%) 5.5% 6.4% 5.8% 6.2% 5.1% 7.3% CV (%) 5.5% 6.4% 5.8% 6.2% 5.1% 7.3%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.014 0.095 0.164 0.272 0.534 0.881 1.457 2.001 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.014 0.095 0.164 0.272 0.534 0.881 1.457 2.001 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.014 0.095 0.164 0.272 0.534 0.881 1.457 2.001 O.D. 0.014 0.095 0.164 0.272 0.534 0.881 1.457 2.001

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.