Human REG1A ELISA Kit PicoKine®

This gene is a type I subclass member of the Reg gene family. It is mapped to 2p12. The Reg gene family is a multigene family grouped into four subclasses, types I, II, III and IV, based on the primary structures of the encoded proteins. This gene encodes a protein that is secreted by the exocrine pancreas. It is associated with islet cell regeneration and diabetogenesis and may be involved in pancreatic lithogenesis. Reg family members REG1B, REGL, PAP and this gene are tandemly clustered on chromosome 2p12 and may have arisen from the same ancestral gene by gene duplication.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 87 91 91 87 89 2.00 2.2% Sample 2 790 831 781 745 786 30.59 3.8% Sample 3 1112 1254 1221 1089 1169 69.96 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 87 91 91 87 89 2.00 2.2% Sample 1 87 91 91 87 89 2.00 2.2% Sample 2 790 831 781 745 786 30.59 3.8% Sample 2 790 831 781 745 786 30.59 3.8% Sample 3 1112 1254 1221 1089 1169 69.96 5.9% Sample 3 1112 1254 1221 1089 1169 69.96 5.9%

Lithostathine-1-alpha; Islet cells regeneration factor; ICRF; Islet of Langerhans regenerating protein; REG; Pancreatic stone protein; PSP; Pancreatic thread protein; PTP; Regenerating islet-derived protein 1-alpha; REG-1-alpha; Regenerating protein I alpha; REG1A; PSPS1

In pancreatic acinar cells and, in lower levels, in brain. Enhanced expression of PSP-related transcripts and intraneuronal accumulation of PSP-like proteins is found in brain from Alzheimer disease and Down syndrome patients.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 89 786 1169 121 845 1307 Standard deviation 4.63 51.09 66.63 8.35 49.01 82.34 CV (%) 5.2% 6.5% 5.7% 6.9% 5.8% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 89 786 1169 121 845 1307 Standard deviation 4.63 51.09 66.63 8.35 49.01 82.34 CV (%) 5.2% 6.5% 5.7% 6.9% 5.8% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 89 786 1169 121 845 1307 Mean (pg/ml) 89 786 1169 121 845 1307 Standard deviation 4.63 51.09 66.63 8.35 49.01 82.34 Standard deviation 4.63 51.09 66.63 8.35 49.01 82.34 CV (%) 5.2% 6.5% 5.7% 6.9% 5.8% 6.3% CV (%) 5.2% 6.5% 5.7% 6.9% 5.8% 6.3%

Might act as an inhibitor of spontaneous calcium carbonate precipitation. May be associated with neuronal sprouting in brain, and with brain and pancreas regeneration.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.044 0.102 0.156 0.293 0.532 0.938 1.577 2.278 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.044 0.102 0.156 0.293 0.532 0.938 1.577 2.278 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.044 0.102 0.156 0.293 0.532 0.938 1.577 2.278 O.D. 0.044 0.102 0.156 0.293 0.532 0.938 1.577 2.278

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.