Human JAM-B/JAM2 ELISA Kit PicoKine®

Junctional adhesion molecule B is a protein that in humans is encoded by the JAM2 gene. It is mapped to 21q21.3. This gene belongs to the immunoglobulin superfamily, and the junctional adhesion molecule (JAM) family. The protein encoded by this gene is a type I membrane protein that is localized at the tight junctions of both epithelial and endothelial cells. It acts as an adhesive ligand for interacting with a variety of immune cell types, and may play a role in lymphocyte homing to secondary lymphoid organs. Alternatively spliced transcript variants have been found for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 27 30 28 30 28 1.29 4.6% Sample 2 190 212 215 192 202 11.32 5.6% Sample 3 408 430 389 450 419 22.92 5.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 27 30 28 30 28 1.29 4.6% Sample 1 27 30 28 30 28 1.29 4.6% Sample 2 190 212 215 192 202 11.32 5.6% Sample 2 190 212 215 192 202 11.32 5.6% Sample 3 408 430 389 450 419 22.92 5.4% Sample 3 408 430 389 450 419 22.92 5.4%

Junctional adhesion molecule B; JAM-B; Junctional adhesion molecule 2; JAM-2; Vascular endothelial junction-associated molecule; VE-JAM; CD322; JAM2; C21orf43; VEJAM; UNQ219/PRO245

Highest expression in the heart, placenta, lung, foreskin and lymph node. Prominently expressed on high endothelial venules, also present on the endothelia of other vessels. Localized to the intercellular boundaries of high endothelial cells.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 27 190 408 31 213 467 Standard deviation 1.13 12.73 22.44 2.11 12.57 28.49 CV (%) 4.2% 6.7% 5.5% 6.8% 5.9% 6.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 27 190 408 31 213 467 Standard deviation 1.13 12.73 22.44 2.11 12.57 28.49 CV (%) 4.2% 6.7% 5.5% 6.8% 5.9% 6.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 27 190 408 31 213 467 Mean (pg/ml) 27 190 408 31 213 467 Standard deviation 1.13 12.73 22.44 2.11 12.57 28.49 Standard deviation 1.13 12.73 22.44 2.11 12.57 28.49 CV (%) 4.2% 6.7% 5.5% 6.8% 5.9% 6.1% CV (%) 4.2% 6.7% 5.5% 6.8% 5.9% 6.1%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.034 0.076 0.103 0.159 0.277 0.567 1.1 1.889 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.034 0.076 0.103 0.159 0.277 0.567 1.1 1.889 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.034 0.076 0.103 0.159 0.277 0.567 1.1 1.889 O.D. 0.034 0.076 0.103 0.159 0.277 0.567 1.1 1.889

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.