Human JAM-B/JAM2 ELISA Kit PicoKine®

Human JAM-B/JAM2 ELISA Kit PicoKine® (96 Tests) . Quantitate Human JAM2 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Junctional adhesion molecule B is a protein that in humans is encoded by the JAM2 gene. It is mapped to 21q21.3. This gene belongs to the immunoglobulin superfamily, and the junctional adhesion molecule (JAM) family. The protein encoded by this gene is a type I membrane protein that is localized at the tight junctions of both epithelial and endothelial cells. It acts as an adhesive ligand for interacting with a variety of immune cell types, and may play a role in lymphocyte homing to secondary lymphoid organs. Alternatively spliced transcript variants have been found for this gene.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 27 30 28 30 28 1.29 4.6% Sample 2 190 212 215 192 202 11.32 5.6% Sample 3 408 430 389 450 419 22.92 5.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 27 30 28 30 28 1.29 4.6% Sample 1 27 30 28 30 28 1.29 4.6% Sample 2 190 212 215 192 202 11.32 5.6% Sample 2 190 212 215 192 202 11.32 5.6% Sample 3 408 430 389 450 419 22.92 5.4% Sample 3 408 430 389 450 419 22.92 5.4%

Synonyms

Junctional adhesion molecule B; JAM-B; Junctional adhesion molecule 2; JAM-2; Vascular endothelial junction-associated molecule; VE-JAM; CD322; JAM2; C21orf43; VEJAM; UNQ219/PRO245

Gene Name

JAM2

Gene ID

58494

UniProt

P57087

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: F29-S238

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Tissue Specificity

Highest expression in the heart, placenta, lung, foreskin and lymph node. Prominently expressed on high endothelial venules, also present on the endothelia of other vessels. Localized to the intercellular boundaries of high endothelial cells.

Applications

ELISA

Field of Research

Cardiovascular, Cell Adhesion, Cell Adhesion Molecules, Cytoskeleton/ECM, Signal Transduction, Tight Junctions, Vasculature

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 27 190 408 31 213 467 Standard deviation 1.13 12.73 22.44 2.11 12.57 28.49 CV (%) 4.2% 6.7% 5.5% 6.8% 5.9% 6.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 27 190 408 31 213 467 Standard deviation 1.13 12.73 22.44 2.11 12.57 28.49 CV (%) 4.2% 6.7% 5.5% 6.8% 5.9% 6.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 27 190 408 31 213 467 Mean (pg/ml) 27 190 408 31 213 467 Standard deviation 1.13 12.73 22.44 2.11 12.57 28.49 Standard deviation 1.13 12.73 22.44 2.11 12.57 28.49 CV (%) 4.2% 6.7% 5.5% 6.8% 5.9% 6.1% CV (%) 4.2% 6.7% 5.5% 6.8% 5.9% 6.1%

Sensitivity

<10 pg/mL

Function

May play a role in the processes of lymphocyte homing to secondary lymphoid organs.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human JAM-B/JAM2

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Junctional adhesion molecule 2

Subcellular Location

Cell membrane, Single-pass type I membrane protein, Tight junction

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.034 0.076 0.103 0.159 0.277 0.567 1.1 1.889 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.034 0.076 0.103 0.159 0.277 0.567 1.1 1.889 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.034 0.076 0.103 0.159 0.277 0.567 1.1 1.889 O.D. 0.034 0.076 0.103 0.159 0.277 0.567 1.1 1.889

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.