Mouse CPE/Carboxypeptidase E ELISA Kit PicoKine®

Mouse CPE ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Cpe in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CPE encodes carboxypeptidase E, a prohormone-processing exopeptidase found in secretory granules of endocrine and neuroendocrine cells. It is mapped to 8 B3.1; 8 32.3 cM. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional enzyme that cleaves the C-terminal basic residues of protein substrates. A missense mutation in this gene is responsible for the obesity phenotype in a mouse model known as the fat mouse. Mice lacking the functional product of this gene exhibit impaired processing of multiple peptide hormones such as proinsulin, prodynorphin, proneurotensin, promelanin-concentrating hormone and pro-opiomelanocortin.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 91 94 82 89 89 4.41 4.9% Sample 2 252 268 294 252 266 17.16 6.4% Sample 3 873 901 1007 933 928 50.04 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 91 94 82 89 89 4.41 4.9% Sample 1 91 94 82 89 89 4.41 4.9% Sample 2 252 268 294 252 266 17.16 6.4% Sample 2 252 268 294 252 266 17.16 6.4% Sample 3 873 901 1007 933 928 50.04 5.3% Sample 3 873 901 1007 933 928 50.04 5.3%

Synonyms

Carboxypeptidase E; CPE; Carboxypeptidase H; CPH; Enkephalin convertase; Prohormone-processing carboxypeptidase

Gene Name

Cpe

Gene ID

12876

UniProt

Q00493

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L43-F476

Capture Antibody

A monoclonal antibody from rat

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Applications

ELISA

Field of Research

Cell Type Marker, Neuron Marker, Neuroscience, Synapse Marker

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 91 252 873 95 258 907 Standard deviation 5.92 12.85 55.00 5.23 17.54 68.03 CV (%) 6.5% 5.1% 6.3% 5.5% 6.8% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 91 252 873 95 258 907 Standard deviation 5.92 12.85 55.00 5.23 17.54 68.03 CV (%) 6.5% 5.1% 6.3% 5.5% 6.8% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 91 252 873 95 258 907 Mean (pg/ml) 91 252 873 95 258 907 Standard deviation 5.92 12.85 55.00 5.23 17.54 68.03 Standard deviation 5.92 12.85 55.00 5.23 17.54 68.03 CV (%) 6.5% 5.1% 6.3% 5.5% 6.8% 7.5% CV (%) 6.5% 5.1% 6.3% 5.5% 6.8% 7.5%

Sensitivity

<50 pg/mL

Function

Removes residual C-terminal Arg or Lys remaining after initial endoprotease cleavage during prohormone processing. Processes proinsulin.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse CPE

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Carboxypeptidase E

Subcellular Location

Secreted, secretory vesicle membrane, Peripheral membrane protein

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.042 0.091 0.137 0.22 0.404 0.671 1.218 2.08 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.042 0.091 0.137 0.22 0.404 0.671 1.218 2.08 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.042 0.091 0.137 0.22 0.404 0.671 1.218 2.08 O.D. 0.042 0.091 0.137 0.22 0.404 0.671 1.218 2.08

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.