Human CPE/Carboxypeptidase E ELISA Kit PicoKine®

Human CPE ELISA Kit PicoKine® (96 Tests) . Quantitate Human CPE in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

This gene encodes a member of the M14 family of metallocarboxypeptidases. It is mapped to 4q32.3. The encoded preproprotein is proteolytically processed to generate the mature peptidase. This peripheral membrane protein cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. This protein may also function independently of its peptidase activity, as a neurotrophic factor that promotes neuronal survival, and as a sorting receptor that binds to regulated secretory pathway proteins, including prohormones. Mutations in this gene are implicated in type 2 diabetes.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 55 51 51 51 52 1.73 3.3% Sample 2 259 231 228 247 241 12.53 5.1% Sample 3 792 756 822 884 813 46.93 5.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 55 51 51 51 52 1.73 3.3% Sample 1 55 51 51 51 52 1.73 3.3% Sample 2 259 231 228 247 241 12.53 5.1% Sample 2 259 231 228 247 241 12.53 5.1% Sample 3 792 756 822 884 813 46.93 5.7% Sample 3 792 756 822 884 813 46.93 5.7%

Synonyms

Carboxypeptidase E; CPE; Carboxypeptidase H; CPH; Enkephalin convertase; Prohormone-processing carboxypeptidase

Gene Name

CPE

Gene ID

1363

UniProt

P16870

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L43-F476

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Tissue Specificity

Isoform 2, but not isoform 1, is overexpressed in hepatocellular carcinoma (at protein level), as well as in other tumors, including pheochromocytomas and paragangliomas.

Applications

ELISA

Field of Research

Cell Type Marker, Neuron Marker, Neuroscience, Synapse Marker

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 55 259 792 60 261 899 Standard deviation 4.02 14.50 34.85 2.76 14.88 61.13 CV (%) 7.3% 5.6% 4.4% 4.6% 5.7% 6.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 55 259 792 60 261 899 Standard deviation 4.02 14.50 34.85 2.76 14.88 61.13 CV (%) 7.3% 5.6% 4.4% 4.6% 5.7% 6.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 55 259 792 60 261 899 Mean (pg/ml) 55 259 792 60 261 899 Standard deviation 4.02 14.50 34.85 2.76 14.88 61.13 Standard deviation 4.02 14.50 34.85 2.76 14.88 61.13 CV (%) 7.3% 5.6% 4.4% 4.6% 5.7% 6.8% CV (%) 7.3% 5.6% 4.4% 4.6% 5.7% 6.8%

Sensitivity

<50 pg/mL

Function

Removes residual C-terminal Arg or Lys remaining after initial endoprotease cleavage during prohormone processing. Processes proinsulin.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human CPE

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Carboxypeptidase E

Subcellular Location

Secreted, secretory vesicle membrane, Peripheral membrane protein, Nucleus

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.002 0.064 0.13 0.271 0.536 1.069 1.818 2.361 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.002 0.064 0.13 0.271 0.536 1.069 1.818 2.361 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.002 0.064 0.13 0.271 0.536 1.069 1.818 2.361 O.D. 0.002 0.064 0.13 0.271 0.536 1.069 1.818 2.361

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.