Rat SerpinE2 ELISA Kit PicoKine®

Rat SerpinE2 ELISA Kit PicoKine® (96 Tests) . Quantitate Rat Serpine2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate), urine. Sensitivity: 0.1ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Glia-derived nexin is a protein that in humans is encoded by the SERPINE2 gene. This gene is mapped to 9q34. SERPINE2 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 386 aminoacids (20-397a.a.) andhaving a molecular mass of 42.9kDa (Molecular size on SDS-PAGE will appear atapproximately 40-57kDa) .SERPINE2 is expressed with a 8 aminoacid His tag atC-Terminus and purified by proprietary chromatographic techniques. This gene displays neurite promoting activity and serine protease inhibitory activity.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 413 438 416 463 432 20.08 4.6% Sample 2 2922 2913 2827 3068 2932 86.57 2.9% Sample 3 9164 8948 9570 8836 9129 280.31 3.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 413 438 416 463 432 20.08 4.6% Sample 1 413 438 416 463 432 20.08 4.6% Sample 2 2922 2913 2827 3068 2932 86.57 2.9% Sample 2 2922 2913 2827 3068 2932 86.57 2.9% Sample 3 9164 8948 9570 8836 9129 280.31 3.0% Sample 3 9164 8948 9570 8836 9129 280.31 3.0%

Synonyms

Glia-derived nexin; GDN; Peptidase inhibitor 7; PI-7; Protease nexin 1; PN-1; Protease nexin I; Serpin E2; Serpine2; Pi7; Pn1

Gene Name

Serpine2

Gene ID

29366

UniProt

P07092

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S20-P397

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Applications

ELISA

Field of Research

Cardiovascular, Cell Adhesion Proteins, Cell Biology, Neurogenesis, Neurology Process, Neuroscience, Proteolysis/Ubiquitin, Proteolytic Enzymes

Standard

50 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate), urine.

Detection Range

0.78 ng/mL - 50 ng/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 413 2922 9164 595 3000 9451 Standard deviation 17.35 131.49 650.64 38.68 165.00 737.18 CV (%) 4.2% 4.5% 7.1% 6.5% 5.5% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 413 2922 9164 595 3000 9451 Standard deviation 17.35 131.49 650.64 38.68 165.00 737.18 CV (%) 4.2% 4.5% 7.1% 6.5% 5.5% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 413 2922 9164 595 3000 9451 Mean (pg/ml) 413 2922 9164 595 3000 9451 Standard deviation 17.35 131.49 650.64 38.68 165.00 737.18 Standard deviation 17.35 131.49 650.64 38.68 165.00 737.18 CV (%) 4.2% 4.5% 7.1% 6.5% 5.5% 7.8% CV (%) 4.2% 4.5% 7.1% 6.5% 5.5% 7.8%

Sensitivity

<0.1 ng/mL

Function

Serine protease inhibitor with activity toward thrombin, trypsin, and urokinase. Promotes neurite extension by inhibiting thrombin. Binds heparin.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant rat SerpinE2

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Serpin family E member 2

Subcellular Location

Extracellular space

Sample Data

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.052 0.099 0.141 0.236 0.399 0.791 1.318 2.094 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.052 0.099 0.141 0.236 0.399 0.791 1.318 2.094 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.052 0.099 0.141 0.236 0.399 0.791 1.318 2.094 O.D. 0.052 0.099 0.141 0.236 0.399 0.791 1.318 2.094

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.