Mouse SerpinE2 ELISA Kit PicoKine®

Mouse SerpinE2 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Serpine2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate), urine. Sensitivity: 0.1ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Glia-derived nexin is a protein that in humans is encoded by the SERPINE2 gene. This gene is mapped to 1 C4; 1 40.97 cM. SERPINE2 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 386 aminoacids (20-397a.a.) andhaving a molecular mass of 42.9kDa (Molecular size on SDS-PAGE will appear atapproximately 40-57kDa) .SERPINE2 is expressed with a 8 aminoacid His tag atC-Terminus and purified by proprietary chromatographic techniques. This gene displays neurite promoting activity and serine protease inhibitory activity.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 599 657 572 652 620 35.83 5.7% Sample 2 2848 2654 2960 2710 2793 119.50 4.2% Sample 3 10128 10127 9926 9025 9801 455.79 4.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 599 657 572 652 620 35.83 5.7% Sample 1 599 657 572 652 620 35.83 5.7% Sample 2 2848 2654 2960 2710 2793 119.50 4.2% Sample 2 2848 2654 2960 2710 2793 119.50 4.2% Sample 3 10128 10127 9926 9025 9801 455.79 4.6% Sample 3 10128 10127 9926 9025 9801 455.79 4.6%

Synonyms

Glia-derived nexin; GDN; Peptidase inhibitor 7; PI-7; Protease nexin 1; PN-1; Protease nexin I; Serine protease-inhibitor 4; Serpin E2; Serpine2; Pi7; Pn1; Spi4

Gene Name

Serpine2

Gene ID

20720

UniProt

Q07235

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S20-P397

Capture Antibody

A polyclonal antibody from goat

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Tissue Specificity

Most abundant in seminal vesicles.

Applications

ELISA

Field of Research

Cardiovascular, Cell Adhesion Proteins, Cell Biology, Neurogenesis, Neurology Process, Neuroscience, Proteolysis/Ubiquitin, Proteolytic Enzymes

Standard

50 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate), urine.

Detection Range

0.78 ng/mL - 50 ng/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 599 2848 10128 604 2990 10197 Standard deviation 33.54 165.18 455.76 36.84 191.36 723.99 CV (%) 5.6% 5.8% 4.5% 6.1% 6.4% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 599 2848 10128 604 2990 10197 Standard deviation 33.54 165.18 455.76 36.84 191.36 723.99 CV (%) 5.6% 5.8% 4.5% 6.1% 6.4% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 599 2848 10128 604 2990 10197 Mean (pg/ml) 599 2848 10128 604 2990 10197 Standard deviation 33.54 165.18 455.76 36.84 191.36 723.99 Standard deviation 33.54 165.18 455.76 36.84 191.36 723.99 CV (%) 5.6% 5.8% 4.5% 6.1% 6.4% 7.1% CV (%) 5.6% 5.8% 4.5% 6.1% 6.4% 7.1%

Sensitivity

<0.1 ng/mL

Function

Serine protease inhibitor with activity toward thrombin, trypsin, and urokinase. Promotes neurite extension by inhibiting thrombin. Binds heparin.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse SerpinE2

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Serine (or cysteine) peptidase inhibitor, clade E, member 2

Subcellular Location

Extracellular space

Sample Data

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.018 0.065 0.123 0.23 0.424 0.716 1.195 1.992 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.018 0.065 0.123 0.23 0.424 0.716 1.195 1.992 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.018 0.065 0.123 0.23 0.424 0.716 1.195 1.992 O.D. 0.018 0.065 0.123 0.23 0.424 0.716 1.195 1.992

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.