Rat Plexin B2/PLXNB2 ELISA Kit PicoKine®

Rat Plexin B2 ELISA Kit PicoKine® (96 Tests) . Quantitate Rat Plxnb2 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 0.1ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Plexin-B2 is a protein that in humans is encoded by the PLXNB2 gene. It is mapped to 7q34. Members of the B class of plexins, such as PLXNB2 are transmembrane receptors that participate in axon guidance and cell migration in response to semaphorins.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1397 1173 1127 1354 1263 59.60 4.7% Sample 2 3219 5387 6069 5685 5090 330.34 6.5% Sample 3 21323 23995 18125 17255 20175 1293.19 6.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1397 1173 1127 1354 1263 59.60 4.7% Sample 1 1397 1173 1127 1354 1263 59.60 4.7% Sample 2 3219 5387 6069 5685 5090 330.34 6.5% Sample 2 3219 5387 6069 5685 5090 330.34 6.5% Sample 3 21323 23995 18125 17255 20175 1293.19 6.4% Sample 3 21323 23995 18125 17255 20175 1293.19 6.4%

Synonyms

Plexin B2; Plxnb2; rCG_59297

Gene Name

Plxnb2

Gene ID

315217

UniProt

D3ZQ57

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: L20-P1202

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Applications

ELISA

Field of Research

Axonal Guidance Proteins, Cell Type Marker, Growth and Development, Neurogenesis, Neurology Process, Neuron Marker, Neuroscience

Standard

50 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

0.78 ng/mL - 50 ng/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1397 3219 21343 1165 4120 14805 Standard deviation 59.37 229.84 1010.71 29.01 174.69 436.75 CV (%) 4.3% 7.1% 4.7% 2.5% 4.2% 3.0% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1397 3219 21343 1165 4120 14805 Standard deviation 59.37 229.84 1010.71 29.01 174.69 436.75 CV (%) 4.3% 7.1% 4.7% 2.5% 4.2% 3.0% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1397 3219 21343 1165 4120 14805 Mean (pg/ml) 1397 3219 21343 1165 4120 14805 Standard deviation 59.37 229.84 1010.71 29.01 174.69 436.75 Standard deviation 59.37 229.84 1010.71 29.01 174.69 436.75 CV (%) 4.3% 7.1% 4.7% 2.5% 4.2% 3.0% CV (%) 4.3% 7.1% 4.7% 2.5% 4.2% 3.0%

Sensitivity

<50 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant rat Plexin B2

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Plexin B2

Subcellular Location

Integral component of plasma membrane

Sample Data

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.029 0.081 0.144 0.254 0.417 0.842 1.327 2.159 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.029 0.081 0.144 0.254 0.417 0.842 1.327 2.159 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.029 0.081 0.144 0.254 0.417 0.842 1.327 2.159 O.D. 0.029 0.081 0.144 0.254 0.417 0.842 1.327 2.159

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.