Mouse Plexin B2/PLXNB2 ELISA Kit PicoKine®

Plexin-B2 is a protein that in humans is encoded by the PLXNB2 gene. It is mapped to 15 E3; 15 44.68 cM. Members of the B class of plexins, such as PLXNB2 are transmembrane receptors that participate in axon guidance and cell migration in response to semaphorins.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 998 1229 829 1435 1123 39.07 3.5% Sample 2 4621 5208 3634 3442 4226 270.48 6.4% Sample 3 20993 24224 12662 13144 17756 458.10 2.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 998 1229 829 1435 1123 39.07 3.5% Sample 1 998 1229 829 1435 1123 39.07 3.5% Sample 2 4621 5208 3634 3442 4226 270.48 6.4% Sample 2 4621 5208 3634 3442 4226 270.48 6.4% Sample 3 20993 24224 12662 13144 17756 458.10 2.6% Sample 3 20993 24224 12662 13144 17756 458.10 2.6%

Detected in macrophages from spleen and bone marrow (at protein level) . Detected in granule cells in the developing cerebellum, dentate gyrus and olfactory bulb.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 998 4621 20993 1294 6131 20908 Standard deviation 26.15 128.93 1280.57 55.25 166.76 1018.22 CV (%) 2.6% 2.8% 6.1% 4.3% 2.7% 4.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 998 4621 20993 1294 6131 20908 Standard deviation 26.15 128.93 1280.57 55.25 166.76 1018.22 CV (%) 2.6% 2.8% 6.1% 4.3% 2.7% 4.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 998 4621 20993 1294 6131 20908 Mean (pg/ml) 998 4621 20993 1294 6131 20908 Standard deviation 26.15 128.93 1280.57 55.25 166.76 1018.22 Standard deviation 26.15 128.93 1280.57 55.25 166.76 1018.22 CV (%) 2.6% 2.8% 6.1% 4.3% 2.7% 4.9% CV (%) 2.6% 2.8% 6.1% 4.3% 2.7% 4.9%

Cell surface receptor for SEMA4C, SEMA4D and SEMA4G that plays an important role in cell-cell signaling. Binding to class 4 semaphorins promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248'. Required for normal differentiation

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.044 0.136 0.185 0.302 0.497 0.893 1.404 1.925 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.044 0.136 0.185 0.302 0.497 0.893 1.404 1.925 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.044 0.136 0.185 0.302 0.497 0.893 1.404 1.925 O.D. 0.044 0.136 0.185 0.302 0.497 0.893 1.404 1.925

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.