Mouse Plexin B2/PLXNB2 ELISA Kit PicoKine®

Mouse Plexin B2 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Plxnb2 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 0.1ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Plexin-B2 is a protein that in humans is encoded by the PLXNB2 gene. It is mapped to 15 E3; 15 44.68 cM. Members of the B class of plexins, such as PLXNB2 are transmembrane receptors that participate in axon guidance and cell migration in response to semaphorins.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 998 1229 829 1435 1123 39.07 3.5% Sample 2 4621 5208 3634 3442 4226 270.48 6.4% Sample 3 20993 24224 12662 13144 17756 458.10 2.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 998 1229 829 1435 1123 39.07 3.5% Sample 1 998 1229 829 1435 1123 39.07 3.5% Sample 2 4621 5208 3634 3442 4226 270.48 6.4% Sample 2 4621 5208 3634 3442 4226 270.48 6.4% Sample 3 20993 24224 12662 13144 17756 458.10 2.6% Sample 3 20993 24224 12662 13144 17756 458.10 2.6%

Synonyms

Plexin-B2; Plxnb2

Gene Name

Plxnb2

Gene ID

140570

UniProt

B2RXS4

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: L20-P1201

Capture Antibody

A monoclonal antibody from rat

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Tissue Specificity

Detected in macrophages from spleen and bone marrow (at protein level) . Detected in granule cells in the developing cerebellum, dentate gyrus and olfactory bulb.

Applications

ELISA

Field of Research

Axonal Guidance Proteins, Cell Type Marker, Growth and Development, Neurogenesis, Neurology Process, Neuron Marker, Neuroscience

Standard

50 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

0.78 ng/mL - 50 ng/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 998 4621 20993 1294 6131 20908 Standard deviation 26.15 128.93 1280.57 55.25 166.76 1018.22 CV (%) 2.6% 2.8% 6.1% 4.3% 2.7% 4.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 998 4621 20993 1294 6131 20908 Standard deviation 26.15 128.93 1280.57 55.25 166.76 1018.22 CV (%) 2.6% 2.8% 6.1% 4.3% 2.7% 4.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 998 4621 20993 1294 6131 20908 Mean (pg/ml) 998 4621 20993 1294 6131 20908 Standard deviation 26.15 128.93 1280.57 55.25 166.76 1018.22 Standard deviation 26.15 128.93 1280.57 55.25 166.76 1018.22 CV (%) 2.6% 2.8% 6.1% 4.3% 2.7% 4.9% CV (%) 2.6% 2.8% 6.1% 4.3% 2.7% 4.9%

Sensitivity

<50 pg/mL

Function

Cell surface receptor for SEMA4C, SEMA4D and SEMA4G that plays an important role in cell-cell signaling. Binding to class 4 semaphorins promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248'. Required for normal differentiation

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse Plexin B2

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Plexin B2

Subcellular Location

Cell membrane, Single-pass type I membrane protein

Sample Data

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.044 0.136 0.185 0.302 0.497 0.893 1.404 1.925 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.044 0.136 0.185 0.302 0.497 0.893 1.404 1.925 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.044 0.136 0.185 0.302 0.497 0.893 1.404 1.925 O.D. 0.044 0.136 0.185 0.302 0.497 0.893 1.404 1.925

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.