Human CTRP9 ELISA Kit PicoKine®

Human CTRP9 ELISA Kit PicoKine® (96 Tests) . Quantitate Human C1QTNF9 in cell culture supernatants, serum and plasma (heparin) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

The C1QTNF9 gene is conserved in chimpanzee, Rhesus monkey, and zebrafish. This gene is mapped to 13q12.12. 42 organisms have orthologs with human gene C1QTNF9.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 65 59 68 62 63 3.35 5.3% Sample 2 280 327 277 285 292 20.26 6.9% Sample 3 1368 1367 1278 1292 1326 41.54 3.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 65 59 68 62 63 3.35 5.3% Sample 1 65 59 68 62 63 3.35 5.3% Sample 2 280 327 277 285 292 20.26 6.9% Sample 2 280 327 277 285 292 20.26 6.9% Sample 3 1368 1367 1278 1292 1326 41.54 3.1% Sample 3 1368 1367 1278 1292 1326 41.54 3.1%

Synonyms

Complement C1q and tumor necrosis factor-related protein 9A; Complement C1q and tumor necrosis factor-related protein 9; C1QTNF9; C1QTNF9A; UNQ6503; PRO21380

Gene Name

C1QTNF9

Gene ID

338872

UniProt

P0C862

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q20-P333

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Tissue Specificity

Expressed predominantly in adipose tissue.

Applications

ELISA

Field of Research

Cytokines, Immunology, Innate Immunity, Secreted Molecules, TNF Superfamily

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin) .

Detection Range

46.9 pg/mL - 3,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 65 280 1368 73 291 1425 Standard deviation 3.19 14.28 79.34 5.18 22.12 111.15 CV (%) 4.9% 5.1% 5.8% 7.1% 7.6% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 65 280 1368 73 291 1425 Standard deviation 3.19 14.28 79.34 5.18 22.12 111.15 CV (%) 4.9% 5.1% 5.8% 7.1% 7.6% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 65 280 1368 73 291 1425 Mean (pg/ml) 65 280 1368 73 291 1425 Standard deviation 3.19 14.28 79.34 5.18 22.12 111.15 Standard deviation 3.19 14.28 79.34 5.18 22.12 111.15 CV (%) 4.9% 5.1% 5.8% 7.1% 7.6% 7.8% CV (%) 4.9% 5.1% 5.8% 7.1% 7.6% 7.8%

Sensitivity

<10 pg/mL

Function

Probable adipokine. Activates AMPK, AKT, and p44/42 MAPK signaling pathways.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human CTRP9

Anticoagulant

Heparin

Gene Name Synonym

C1q and TNF related 9

Subcellular Location

Secreted

Sample Data

Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.024 0.117 0.193 0.372 0.646 1.049 1.787 2.421 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.024 0.117 0.193 0.372 0.646 1.049 1.787 2.421 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.024 0.117 0.193 0.372 0.646 1.049 1.787 2.421 O.D. 0.024 0.117 0.193 0.372 0.646 1.049 1.787 2.421

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.