Human RET ELISA Kit PicoKine®

Human RET ELISA Kit PicoKine® (96 Tests) . Quantitate Human RET in cell culture supernatants, serum and plasma (heparin) . Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

This gene encodes a transmembrane receptor and member of the tyrosine protein kinase family of proteins. It is mapped to 10q11.21. Binding of ligands such as GDNF (glial cell-line derived neurotrophic factor) and other related proteins to the encoded receptor stimulates receptor dimerization and activation of downstream signaling pathways that play a role in cell differentiation, growth, migration and survival. The encoded receptor is important in development of the nervous system, and the development of organs and tissues derived from the neural crest. This proto-oncogene can undergo oncogenic activation through both cytogenetic rearrangement and activating point mutations. Mutations in this gene are associated with Hirschsprung disease and central hypoventilation syndrome and have been identified in patients with renal agenesis.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 148 147 153 148 149 2.34 1.5% Sample 2 628 656 611 669 641 22.79 3.5% Sample 3 2813 3203 3280 2837 3033 210.19 6.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 148 147 153 148 149 2.34 1.5% Sample 1 148 147 153 148 149 2.34 1.5% Sample 2 628 656 611 669 641 22.79 3.5% Sample 2 628 656 611 669 641 22.79 3.5% Sample 3 2813 3203 3280 2837 3033 210.19 6.9% Sample 3 2813 3203 3280 2837 3033 210.19 6.9%

Synonyms

Proto-oncogene tyrosine-protein kinase receptor Ret; Cadherin family member 12; Proto-oncogene c-Ret; RET; CDHF12; CDHR16; PTC; RET51

Gene Name

RET

Gene ID

5979

UniProt

P07949

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L29-R635

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Applications

ELISA

Field of Research

Cancer, Oncoproteins, Oncoproteins/Suppressors, Protein Phosphorylation, Receptor Tyrosine Kinases, Signal Transduction, Tyrosine Kinases

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin) .

Detection Range

93.8 pg/mL - 6,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 148 628 2813 162 691 2933 Standard deviation 6.96 32.03 149.09 9.72 44.92 231.71 CV (%) 4.7% 5.1% 5.3% 6.0% 6.5% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 148 628 2813 162 691 2933 Standard deviation 6.96 32.03 149.09 9.72 44.92 231.71 CV (%) 4.7% 5.1% 5.3% 6.0% 6.5% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 148 628 2813 162 691 2933 Mean (pg/ml) 148 628 2813 162 691 2933 Standard deviation 6.96 32.03 149.09 9.72 44.92 231.71 Standard deviation 6.96 32.03 149.09 9.72 44.92 231.71 CV (%) 4.7% 5.1% 5.3% 6.0% 6.5% 7.9% CV (%) 4.7% 5.1% 5.3% 6.0% 6.5% 7.9%

Sensitivity

<15 pg/mL

Function

Receptor tyrosine-protein kinase involved in numerous cellular mechanisms including cell proliferation, neuronal navigation, cell migration, and cell differentiation upon binding with glial cell derived neurotrophic factor family ligands. Phosphorylates P

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human RET

Anticoagulant

Heparin

Gene Name Synonym

Ret proto-oncogene

Subcellular Location

Endosome membrane, Single-pass type I membrane protein, Cell membrane

Sample Data

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.013 0.09 0.158 0.284 0.572 0.968 1.583 2.158 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.013 0.09 0.158 0.284 0.572 0.968 1.583 2.158 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.013 0.09 0.158 0.284 0.572 0.968 1.583 2.158 O.D. 0.013 0.09 0.158 0.284 0.572 0.968 1.583 2.158

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.