Rat GAS6 ELISA Kit PicoKine®

GAS6 (Growth arrest-specific 6), also known as AXLLG or AXSF, is a human gene coding for the Gas6 protein. It is mapped to 13q34. GAS6 was originally found as a gene upregulated by growth arrested fibroblasts. This gene product is a gamma-carboxyglutamic acid (Gla) -containing protein which thought to be involved in the stimulation of cell proliferation, and may play a role in thrombosis. What's more, the product of the GAS6 gene, a ligand for the AXL, MER, and TYRO3 tyrosine kinase receptors, is a vitamin K-dependent protein, structurally related to anticoagulant protein S but lacking its anticoagulant activity. This protein also shows great importance in the cardiovascular system.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 73 66 71 70 70 2.54 3.6% Sample 2 308 302 310 268 297 17.00 5.7% Sample 3 856 806 872 876 852 27.87 3.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 73 66 71 70 70 2.54 3.6% Sample 1 73 66 71 70 70 2.54 3.6% Sample 2 308 302 310 268 297 17.00 5.7% Sample 2 308 302 310 268 297 17.00 5.7% Sample 3 856 806 872 876 852 27.87 3.2% Sample 3 856 806 872 876 852 27.87 3.2%

Atherosclerosis, Cardiovascular, Hematopoietic Progenitors, Mesenchymal Stem Cells, Myeloid, Protein Phosphorylation, Receptor Tyrosine Kinases, Signal Transduction, Stem Cells, Tyrosine Kinases

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 73 308 856 75 321 969 Standard deviation 3.94 13.24 48.79 4.65 20.22 73.64 CV (%) 5.4% 4.3% 5.7% 6.2% 6.3% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 73 308 856 75 321 969 Standard deviation 3.94 13.24 48.79 4.65 20.22 73.64 CV (%) 5.4% 4.3% 5.7% 6.2% 6.3% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 73 308 856 75 321 969 Mean (pg/ml) 73 308 856 75 321 969 Standard deviation 3.94 13.24 48.79 4.65 20.22 73.64 Standard deviation 3.94 13.24 48.79 4.65 20.22 73.64 CV (%) 5.4% 4.3% 5.7% 6.2% 6.3% 7.6% CV (%) 5.4% 4.3% 5.7% 6.2% 6.3% 7.6%

Ligand for tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.004 0.076 0.122 0.217 0.362 0.639 1.209 1.952 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.004 0.076 0.122 0.217 0.362 0.639 1.209 1.952 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.004 0.076 0.122 0.217 0.362 0.639 1.209 1.952 O.D. 0.004 0.076 0.122 0.217 0.362 0.639 1.209 1.952

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.