Human JAM-C ELISA Kit PicoKine®

This gene is mapped to 11q25. Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. Unlike other proteins in this family, the this protein is unable to adhere to leukocyte cell lines and only forms weak homotypic interactions. The encoded protein is a member of the junctional adhesion molecule protein family and acts as a receptor for another member of this family. A mutation in an intron of this gene is associated with hemorrhagic destruction of the brain, subependymal calcification, and congenital cataracts. Alternative splicing results in multiple transcript variants.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 90 96 100 99 96 3.89 4.0% Sample 2 422 347 363 385 379 28.12 7.4% Sample 3 1408 1509 1433 1363 1428 52.94 3.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 90 96 100 99 96 3.89 4.0% Sample 1 90 96 100 99 96 3.89 4.0% Sample 2 422 347 363 385 379 28.12 7.4% Sample 2 422 347 363 385 379 28.12 7.4% Sample 3 1408 1509 1433 1363 1428 52.94 3.7% Sample 3 1408 1509 1433 1363 1428 52.94 3.7%

Highest expression in placenta, brain and kidney. Significant expression is detected on platelets. Expressed in intestinal mucosa cells. Expressed in the vascular endothelium. Found in serum. Also detected in the synovial fluid of patients with rheumatoid

Atherosclerosis, Cardiovascular, Cell Adhesion, Cell Adhesion Molecules, Cell Type Markers, Cytoskeleton/ECM, Platelets, Signal Transduction, Tags & Cell Markers, Vascular Inflammation, Vasculature

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 90 422 1408 92 430 1466 Standard deviation 4.86 23.63 57.73 4.32 27.09 104.09 CV (%) 5.4% 5.6% 4.1% 4.7% 6.3% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 90 422 1408 92 430 1466 Standard deviation 4.86 23.63 57.73 4.32 27.09 104.09 CV (%) 5.4% 5.6% 4.1% 4.7% 6.3% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 90 422 1408 92 430 1466 Mean (pg/ml) 90 422 1408 92 430 1466 Standard deviation 4.86 23.63 57.73 4.32 27.09 104.09 Standard deviation 4.86 23.63 57.73 4.32 27.09 104.09 CV (%) 5.4% 5.6% 4.1% 4.7% 6.3% 7.1% CV (%) 5.4% 5.6% 4.1% 4.7% 6.3% 7.1%

Participates in cell-cell adhesion. It is a counter-receptor for ITGAM, mediating leukocyte-platelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN) . The soluble form is a mediator of angi

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.035 0.119 0.173 0.253 0.433 0.84 1.379 2.057 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.035 0.119 0.173 0.253 0.433 0.84 1.379 2.057 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.035 0.119 0.173 0.253 0.433 0.84 1.379 2.057 O.D. 0.035 0.119 0.173 0.253 0.433 0.84 1.379 2.057

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.