Human CPA2 ELISA Kit PicoKine®

Human CPA2 ELISA Kit PicoKine® (96 Tests) . Quantitate Human CPA2 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Carboxypeptidase A2 is an enzyme that in humans is encoded by the CPA2 gene. This gene is mapped to 7q32.2. Three different forms of human pancreatic procarboxypeptidase A have been isolated. The encoded protein represents the A2 form, which is a monomeric protein with different biochemical properties from the A1 and A3 forms. The A2 form of pancreatic procarboxypeptidase acts on aromatic C-terminal residues and is a secreted protein.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 108 117 106 116 111 4.81 4.3% Sample 2 747 740 627 672 696 49.68 7.1% Sample 3 2199 2420 2342 2125 2271 115.91 5.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 108 117 106 116 111 4.81 4.3% Sample 1 108 117 106 116 111 4.81 4.3% Sample 2 747 740 627 672 696 49.68 7.1% Sample 2 747 740 627 672 696 49.68 7.1% Sample 3 2199 2420 2342 2125 2271 115.91 5.1% Sample 3 2199 2420 2342 2125 2271 115.91 5.1%

Synonyms

Carboxypeptidase A2; CPA2

Gene Name

CPA2

Gene ID

1358

UniProt

P48052

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S115-Y419

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Applications

ELISA

Field of Research

Cell Biology, Proteolysis/Ubiquitin, Proteolytic Enzymes

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

93.6 pg/mL - 6,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 108 747 2199 162 749 2549 Standard deviation 4.75 34.36 112.15 10.69 53.93 188.63 CV (%) 4.4% 4.6% 5.1% 6.6% 7.2% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 108 747 2199 162 749 2549 Standard deviation 4.75 34.36 112.15 10.69 53.93 188.63 CV (%) 4.4% 4.6% 5.1% 6.6% 7.2% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 108 747 2199 162 749 2549 Mean (pg/ml) 108 747 2199 162 749 2549 Standard deviation 4.75 34.36 112.15 10.69 53.93 188.63 Standard deviation 4.75 34.36 112.15 10.69 53.93 188.63 CV (%) 4.4% 4.6% 5.1% 6.6% 7.2% 7.4% CV (%) 4.4% 4.6% 5.1% 6.6% 7.2% 7.4%

Sensitivity

<15 pg/mL

Function

Similar to that of carboxypeptidase A (EC 3.4.17.1), but with a preference for bulkier C-terminal residues.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human CPA2

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Carboxypeptidase A2

Subcellular Location

Secreted

Sample Data

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.02 0.058 0.098 0.192 0.392 0.775 1.429 2.01 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.02 0.058 0.098 0.192 0.392 0.775 1.429 2.01 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.02 0.058 0.098 0.192 0.392 0.775 1.429 2.01 O.D. 0.02 0.058 0.098 0.192 0.392 0.775 1.429 2.01

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.