Human SEZ6L ELISA Kit PicoKine®

Human SEZ6L ELISA Kit PicoKine® (96 Tests) . Quantitate Human SEZ6L in cell culture supernatants, serum and plasma (heparin) . Sensitivity: 0.1ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Seizure 6-like protein is a protein that in humans is encoded by the SEZ6L gene. This gene is mapped to 22q12.1.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 489 478 517 524 502 19.06 3.7% Sample 2 1734 1756 1906 1797 1798 66.19 3.6% Sample 3 10155 8845 9766 8917 9420 557.57 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 489 478 517 524 502 19.06 3.7% Sample 1 489 478 517 524 502 19.06 3.7% Sample 2 1734 1756 1906 1797 1798 66.19 3.6% Sample 2 1734 1756 1906 1797 1798 66.19 3.6% Sample 3 10155 8845 9766 8917 9420 557.57 5.9% Sample 3 10155 8845 9766 8917 9420 557.57 5.9%

Synonyms

Seizure 6-like protein; SEZ6L; KIAA0927; UNQ2542/PRO6094

Gene Name

SEZ6L

Gene ID

23544

UniProt

Q9BYH1

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L29-I1024

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Tissue Specificity

Widely expressed, including adult and fetal brains and lungs. Not expressed in all lung cancer cell lines.

Applications

ELISA

Field of Research

Cancer, Intracellular Signaling, Neuroscience, Neurotransmission, Oncoproteins/Suppressors, Secretory Vesicles, Tumor Suppressors

Standard

20 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin) .

Detection Range

0.312 ng/mL - 20 ng/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 489 1734 10155 544 2012 11046 Standard deviation 31.30 97.10 710.85 23.39 114.68 839.50 CV (%) 6.4% 5.6% 7.0% 4.3% 5.7% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 489 1734 10155 544 2012 11046 Standard deviation 31.30 97.10 710.85 23.39 114.68 839.50 CV (%) 6.4% 5.6% 7.0% 4.3% 5.7% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 489 1734 10155 544 2012 11046 Mean (pg/ml) 489 1734 10155 544 2012 11046 Standard deviation 31.30 97.10 710.85 23.39 114.68 839.50 Standard deviation 31.30 97.10 710.85 23.39 114.68 839.50 CV (%) 6.4% 5.6% 7.0% 4.3% 5.7% 7.6% CV (%) 6.4% 5.6% 7.0% 4.3% 5.7% 7.6%

Sensitivity

<0.1 ng/mL

Function

May contribute to specialized endoplasmic reticulum functions in neurons.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human SEZ6L

Anticoagulant

Heparin

Gene Name Synonym

Seizure related 6 homolog like

Subcellular Location

Endoplasmic reticulum membrane, Single-pass type I membrane protein

Sample Data

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.05 0.126 0.17 0.267 0.535 0.876 1.383 1.964 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.05 0.126 0.17 0.267 0.535 0.876 1.383 1.964 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.05 0.126 0.17 0.267 0.535 0.876 1.383 1.964 O.D. 0.05 0.126 0.17 0.267 0.535 0.876 1.383 1.964

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.