Rat Plexin A1/PLXNA1 ELISA Kit PicoKine®

Rat Plexin A1 ELISA Kit PicoKine® (96 Tests) . Quantitate Rat Plxna1 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 0.1ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Plexin-A1 is a protein that in humans is encoded by the PLXNA1 gene. This gene is mapped to 4q34. Plexin A1 has been shown to interact with AKT1.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 525 574 531 506 534 24.86 4.6% Sample 2 2481 2577 2752 2419 2557 125.74 4.9% Sample 3 8381 9525 9678 8669 9063 550.46 6.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 525 574 531 506 534 24.86 4.6% Sample 1 525 574 531 506 534 24.86 4.6% Sample 2 2481 2577 2752 2419 2557 125.74 4.9% Sample 2 2481 2577 2752 2419 2557 125.74 4.9% Sample 3 8381 9525 9678 8669 9063 550.46 6.0% Sample 3 8381 9525 9678 8669 9063 550.46 6.0%

Synonyms

Plexin A1; RCG56095; Plxna1; RGD1560871; rCG_56095

Gene Name

Plxna1

Gene ID

362398

UniProt

D3Z981

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S46-S1255

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Applications

ELISA

Field of Research

Axonal Guidance Proteins, Growth and Development, More Gpcr, Neurogenesis, Neurology Process, Neuroscience, Neurotransmission, Receptors / Channels

Standard

20 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

0.312 ng/mL - 20 ng/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 525 2481 8381 569 2489 9641 Standard deviation 28.35 151.34 645.34 27.88 136.90 655.59 CV (%) 5.4% 6.1% 7.7% 4.9% 5.5% 6.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 525 2481 8381 569 2489 9641 Standard deviation 28.35 151.34 645.34 27.88 136.90 655.59 CV (%) 5.4% 6.1% 7.7% 4.9% 5.5% 6.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 525 2481 8381 569 2489 9641 Mean (pg/ml) 525 2481 8381 569 2489 9641 Standard deviation 28.35 151.34 645.34 27.88 136.90 655.59 Standard deviation 28.35 151.34 645.34 27.88 136.90 655.59 CV (%) 5.4% 6.1% 7.7% 4.9% 5.5% 6.8% CV (%) 5.4% 6.1% 7.7% 4.9% 5.5% 6.8%

Sensitivity

<0.1 ng/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant rat Plexin A1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Plexin A1

Subcellular Location

Integral component of membrane

Sample Data

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.043 0.133 0.176 0.258 0.449 0.857 1.573 2.181 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.043 0.133 0.176 0.258 0.449 0.857 1.573 2.181 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.043 0.133 0.176 0.258 0.449 0.857 1.573 2.181 O.D. 0.043 0.133 0.176 0.258 0.449 0.857 1.573 2.181

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.